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Product Info Summary
| SKU: | A00119S71 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Rac1/Cdc42 (Phospho-Ser71) Antibody
SKU/Catalog Number
A00119S71
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rac1/Cdc42 (Phospho-Ser71) Antibody catalog # A00119S71. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rac1/Cdc42 (Phospho-Ser71) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00119S71)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser71 of human CDC42.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00119S71 is reactive to CDC42 in Human
Reconstitution
Calculated molecular weight
21259 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00119S71 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of CDC42 using Anti-Rac1/Cdc42 (Phospho-Ser71) Antibody (A00119S71).
ICC staining Phospho-Rac1+Cdc42(Ser71) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 2. Immunocytochemistry staining of CDC42 using Anti-Rac1/Cdc42 (Phospho-Ser71) Antibody (A00119S71).
ICC staining Phospho-Rac1+Cdc42(Ser71) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For CDC42 (Source: Uniprot.org, NCBI)
Gene Name
CDC42
Full Name
Cell division control protein 42 homolog
Weight
21259 MW
Superfamily
small GTPase superfamily
Alternative Names
Cell division control protein 42 homolog;G25K GTP-binding protein;CDC42; CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42, check out the CDC42 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rac1/Cdc42 (Phospho-Ser71) Antibody (A00119S71)
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