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Product Info Summary
| SKU: | A01742 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Protein C/PROC Antibody Picoband®
View all Coagulation Factor XIV/Protein C Antibodies
SKU/Catalog Number
A01742
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein C/PROC Antibody Picoband® catalog # A01742. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein C/PROC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01742)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Protein C recombinant protein (Position: D200-P461).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01742 is reactive to PROC in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.071kDa
Background of Coagulation Factor XIV/Protein C
Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped on 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01742 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
ELISA,0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Protein C using anti-Protein C antibody (A01742).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody (Catalog # A01742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD.
Protein Target Info & Infographic
Gene/Protein Information For PROC (Source: Uniprot.org, NCBI)
Gene Name
PROC
Full Name
Vitamin K-dependent protein C
Weight
52.071kDa
Superfamily
peptidase S1 family
Alternative Names
Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; Vitamin K-dependent protein C light chain; Vitamin K-dependent protein C heavy chain; Activation peptide; PROC PROC APC, PC1, THPH3, THPH4, PROC protein C, inactivator of coagulation factors Va and VIIIa vitamin K-dependent protein C|Protein C-Nagoya|activated protein C|anticoagulant protein C|autoprothrombin IIA|blood coagulation factor XIV|prepro-protein C|type I protein C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROC, check out the PROC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein C/PROC Antibody Picoband® (A01742)
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