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Product Info Summary
| SKU: | A00807Y497 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody
View all IL-4R alpha Antibodies
SKU/Catalog Number
A00807Y497
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody catalog # A00807Y497. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00807Y497)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IL-4R/CD124 around the phosphorylation site of Tyr497. AA range:463-512
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00807Y497 is reactive to IL4R in Human, Mouse
Reconstitution
Calculated molecular weight
89658 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00807Y497 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using Phospho-IL-4Rα (Y497) polyclonal antibody
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Immunofluorescence analysis of HeLa cells, using IL-4R/CD124 (Phospho-Tyr497) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells, using IL-4R/CD124 (Phospho-Tyr497) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For IL4R (Source: Uniprot.org, NCBI)
Gene Name
IL4R
Full Name
Interleukin-4 receptor subunit alpha
Weight
89658 MW
Superfamily
type I cytokine receptor family
Alternative Names
Interleukin-4 receptor subunit alpha;IL-4 receptor subunit alpha;IL-4R subunit alpha;IL-4R-alpha;IL-4RA;CD124;Soluble interleukin-4 receptor subunit alpha;Soluble IL-4 receptor subunit alpha;Soluble IL-4R-alpha;sIL4Ralpha/prot;IL-4-binding protein;IL4-BP;IL4R;IL4RA;582J2.1; IL4R CD124, IL-4RAA, IL4R interleukin 4 receptor interleukin-4 receptor subunit alpha|IL-4 receptor subunit alpha|IL4R nirs variant 1|interleukin 13 receptor|interleukin-4 receptor alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL4R, check out the IL4R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL4R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody (A00807Y497)
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