Click image to see more details
Product Info Summary
| SKU: | PB9013 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL2 Antibody Picoband®
SKU/Catalog Number
PB9013
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL2 Antibody Picoband® catalog # PB9013. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9013)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IL-2 recombinant protein (Position: A21-Q155). Rat IL-2 shares 66% and 80% amino acid (aa) sequences identity with human and mouse IL-2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9013 is reactive to Il2 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
17633 MW
Background of IL-2
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. This gene for interleukin 2 (IL2) is assigned to chromosome 4. IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9013 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: Rat Thymus Tissue, Rat Liver Tissue, NRK Whole Cell, PC12 Whole Cell, RH35 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL2 using anti-IL2 antibody (PB9013).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Thymus Tissue Lysate,
Lane 2: Rat Liver Tissue Lysate,
Lane 3: NRK Whole Cell Lysate,
Lane 4: PC12 Whole Cell Lysate,
Lane 5: RH35 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (Catalog # PB9013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 at approximately 18KD. The expected band size for IL2 is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For Il2 (Source: Uniprot.org, NCBI)
Gene Name
Il2
Full Name
Interleukin-2
Weight
17633 MW
Superfamily
IL-2 family
Alternative Names
Interleukin-2;IL-2;T-cell growth factor;TCGF;Il2;Il-2; IL2 IL-2, TCGF, lymphokine interleukin 2 interleukin-2|T cell growth factor|aldesleukin|involved in regulation of T-cell clonal expansion
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il2, check out the Il2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL2 Antibody Picoband® (PB9013)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IL2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-IL2 Antibody Picoband®
Question
you antibody using your anti-IL2 antibody for positive regulation of activated t cell proliferation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
We appreciate your inquiry. This PB9013 anti-IL2 antibody is tested on rat thymus tissue, tissue lysate, liver tissue, nrk whole cell lysate, pc12 whole cell lysate, rh35 whole cell lysate. It is guaranteed to work for WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-16
Question
We have observed staining in rat placenta. Do you have any suggestions? Is anti-IL2 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
From literature placenta does express IL2. From Uniprot.org, IL2 is expressed in small intestine peyer's patch, placenta, blood, among other tissues. Regarding which tissues have IL2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Placenta, Pubmed ID: 8824916
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-IL2 antibody PB9013 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-IL2 antibody (PB9013) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Our lab were well pleased with the WB result of your anti-IL2 antibody. However we have seen positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
According to literature, blood does express IL2. Generally IL2 expresses in secreted. Regarding which tissues have IL2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Placenta, Pubmed ID: 8824916
Boster Scientific Support
Answered: 2019-09-17
