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Product Info Summary
| SKU: | A00807-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC |
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Product info
Product Name
Anti-IL4R Antibody
View all IL-4R alpha Antibodies
SKU/Catalog Number
A00807-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL4R Antibody catalog # A00807-2. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL4R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00807-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IL4R recombinant protein (Position: I26-E719).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00807-2 is reactive to Il4r in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
180 kDa
Calculated molecular weight
89.658kDa
Background of IL-4R alpha
IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00807-2 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml,
Positive Control
IHC: rat spleen tissue
FCM: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of IL4R using anti-IL4R antibody (A00807-2).
IL4R was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL4R Antibody (A00807-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Flow Cytometry analysis of mouse spleen tissue using anti-IL4R antibody (A00807-2).
Overlay histogram showing mouse spleen tissue cells stained with A00807-2 (Blue line). The tissue section was fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL4R Antibody (A00807-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Il4r (Source: Uniprot.org, NCBI)
Gene Name
Il4r
Full Name
Interleukin-4 receptor subunit alpha
Weight
89.658kDa
Superfamily
type I cytokine receptor family
Alternative Names
Oncostatin-M; OSM; Osm IL4R CD124, IL-4RAA, IL4R interleukin 4 receptor interleukin-4 receptor subunit alpha|IL-4 receptor subunit alpha|IL4R nirs variant 1|interleukin 13 receptor|interleukin-4 receptor alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il4r, check out the Il4r Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il4r: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL4R Antibody (A00807-2)
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