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Product Info Summary
| SKU: | A00807-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IL4R Antibody (C-term)
View all IL-4R alpha Antibodies
SKU/Catalog Number
A00807-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-IL4R Antibody (C-term) (Catalog # A00807-3). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IL4R Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00807-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This IL4R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 677-691 amino acids from the C-terminal region of human IL4R.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00807-3 is reactive to IL4R in Human
Reconstitution
Calculated molecular weight
89658 Da
Background of IL-4R alpha
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00807-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody (C-term). A00807-3 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Flow cytometric analysis of Ramos cells using IL4R Antibody (C-term)(green, ) compared to an isotype control of rabbit IgG(blue). A00807-3 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For IL4R (Source: Uniprot.org, NCBI)
Gene Name
IL4R
Full Name
Interleukin-4 receptor subunit alpha
Weight
89658 Da
Superfamily
type I cytokine receptor family
Alternative Names
Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, Soluble interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP, IL4R, IL4RA IL4R CD124, IL-4RAA, IL4R interleukin 4 receptor interleukin-4 receptor subunit alpha|IL-4 receptor subunit alpha|IL4R nirs variant 1|interleukin 13 receptor|interleukin-4 receptor alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL4R, check out the IL4R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL4R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL4R Antibody (C-term) (A00807-3)
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