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Product Info Summary
| SKU: | A12257 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PHKG1 Antibody
SKU/Catalog Number
A12257
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PHKG1 Antibody catalog # A12257. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHKG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12257)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PHKG1. AA range:241-290
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12257 is reactive to PHKG1 in Human
Reconstitution
Calculated molecular weight
45024 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12257 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HuvEc cells using PHKG1 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using PHKG1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PHKG1 (Source: Uniprot.org, NCBI)
Gene Name
PHKG1
Full Name
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
Weight
45024 MW
Superfamily
protein kinase superfamily
Alternative Names
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform;PHK-gamma-M;2.7.11.19;Phosphorylase kinase subunit gamma-1;Serine/threonine-protein kinase PHKG1;2.7.11.1;2.7.11.26;PHKG1;PHKG; PHKG1 PHKG phosphorylase kinase catalytic subunit gamma 1 phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform|PHK-gamma-M|phosphorylase kinase gamma subunit 1|phosphorylase kinase subunit gamma-1|phosphorylase kinase, gamma 1 (muscle)|serine/threonine-protein kinase PHKG1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHKG1, check out the PHKG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHKG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHKG1 Antibody (A12257)
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No publications found for A12257
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PHKG1 Antibody
Question
Would A12257 anti-PHKG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
It shows on the product datasheet, A12257 anti-PHKG1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-02
Question
Is this A12257 anti-PHKG1 antibody reactive to the isotypes of PHKG1?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The immunogen of A12257 anti-PHKG1 antibody is Synthesized peptide derived from the Internal region of human PHKG1. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-09
Question
We are currently using anti-PHKG1 antibody A12257 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
The anti-PHKG1 antibody (A12257) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-20
Question
We want to test anti-PHKG1 antibody A12257 on human muscle of leg for research purposes, then I may be interested in using anti-PHKG1 antibody A12257 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Evans
Verified customer
Asked: 2013-09-03
Answer
The products we sell, including anti-PHKG1 antibody A12257, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-09-03
