Click image to see more details
Product Info Summary
| SKU: | PA1987 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TORC1/CRTC1 Antibody Picoband®
SKU/Catalog Number
PA1987
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TORC1/CRTC1 Antibody catalog # PA1987. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TORC1/CRTC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1987)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TORC1, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1987 is reactive to CRTC1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
67300 MW
Background of TORC1
CRTC1 (CREB-Regulated Transcription Coactivator 1), also known as MECT1, TORC1 or KIAA0616, is a protein that in humans is encoded by the CRTC1 gene. By sequence analysis, Tonon et al. (2003) mapped the CRTC1 gene to chromosome 19p13. Kovacs et al. (2007) found that Torc1 was expressed in adult mouse brain and cultured neurons, and that it translocated to the nucleus upon concomitant activation of calcium and cAMP signaling pathways. Mair et al. (2011) demonstrated that CRTC1 is a direct AMPK target, and interacts with the CREB homolog-1 (CRH1) transcription factor in vivo. The prolongevity effects of activating AMPK or deactivating calcineurin decrease CRTC1 and CRH1 activity and induce transcriptional responses similar to those of CRH1-null worms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1987 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TORC1 using anti-TORC1 antibody (PA1987).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1 antigen affinity purified polyclonal antibody (Catalog # PA1987) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC1 at approximately 78KD. The expected band size for TORC1 is at 67KD.
Protein Target Info & Infographic
Gene/Protein Information For CRTC1 (Source: Uniprot.org, NCBI)
Gene Name
CRTC1
Full Name
CREB-regulated transcription coactivator 1
Weight
67300 MW
Superfamily
TORC family
Alternative Names
CREB-regulated transcription coactivator 1;Mucoepidermoid carcinoma translocated protein 1;Transducer of regulated cAMP response element-binding protein 1;TORC-1;Transducer of CREB protein 1;CRTC1 ;KIAA0616 , MECT1 , TORC1 , WAMTP1 ; CRTC1 MAML2, MECT1, Mam-2, TORC-1, TORC1, WAMTP1 CREB regulated transcription coactivator 1 CREB-regulated transcription coactivator 1|Mastermind-like protein 2|mucoepidermoid carcinoma translocated protein 1|transducer of regulated cAMP response element-binding protein (CREB) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRTC1, check out the CRTC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRTC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TORC1/CRTC1 Antibody Picoband® (PA1987)
Hello CJ!
No publications found for PA1987
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TORC1/CRTC1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TORC1/CRTC1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-TORC1/CRTC1 Antibody Picoband®
Question
I see that the anti-TORC1/CRTC1 antibody PA1987 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-26
Question
Here is the WB image, lot number and protocol we used for dorsal plus ventral thalamus using anti-TORC1/CRTC1 antibody PA1987. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-20
Question
We are currently using anti-TORC1/CRTC1 antibody PA1987 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on pig tissues as well?
E. Parker
Verified customer
Asked: 2015-08-06
Answer
The anti-TORC1/CRTC1 antibody (PA1987) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-06
Question
Does PA1987 anti-TORC1/CRTC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Walker
Verified customer
Asked: 2014-01-29
Answer
You can see on the product datasheet, PA1987 anti-TORC1/CRTC1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-01-29
