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Product Info Summary
| SKU: | A01951-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-TORC1/CRTC1 Antibody Picoband®
SKU/Catalog Number
A01951-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TORC1/CRTC1 Antibody Picoband® catalog # A01951-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TORC1/CRTC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01951-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TORC1/CRTC1 recombinant protein (Position: E30-E568).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01951-2 is reactive to CRTC1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
67.3kDa
Background of TORC1
CRTC1 (CREB-Regulated Transcription Coactivator 1), also known as MECT1, TORC1 or KIAA0616, is a protein that in humans is encoded by the CRTC1 gene. By sequence analysis, Tonon et al. (2003) mapped the CRTC1 gene to chromosome 19p13. Kovacs et al. (2007) found that Torc1 was expressed in adult mouse brain and cultured neurons, and that it translocated to the nucleus upon concomitant activation of calcium and cAMP signaling pathways. Mair et al. (2011) demonstrated that CRTC1 is a direct AMPK target, and interacts with the CREB homolog-1 (CRH1) transcription factor in vivo. The prolongevity effects of activating AMPK or deactivating calcineurin decrease CRTC1 and CRH1 activity and induce transcriptional responses similar to those of CRH1-null worms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01951-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue, human Hela whole cell
IHC: mouse brain tissue, rat brain tissue, human glioma tissue
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1/CRTC1 antigen affinity purified polyclonal antibody (Catalog # A01951-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC1/CRTC1 at approximately 78KD. The expected band size for TORC1/CRTC1 is at 78KD.
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Figure 2. IHC analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2).
TORC1/CRTC1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TORC1/CRTC1 Antibody (A01951-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2).
TORC1/CRTC1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TORC1/CRTC1 Antibody (A01951-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of U20S cells using anti-TORC1/CRTC1 antibody (A01951-2).
Overlay histogram showing U20S cells stained with A01951-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TORC1/CRTC1 Antibody (A01951-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 5. IHC analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2).
TORC1/CRTC1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TORC1/CRTC1 Antibody (A01951-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CRTC1 (Source: Uniprot.org, NCBI)
Gene Name
CRTC1
Full Name
CREB-regulated transcription coactivator 1
Weight
67.3kDa
Superfamily
TORC family
Alternative Names
CREB-regulated transcription coactivator 1; Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1; TORC-1; Transducer of CREB protein 1; CRTC1; KIAA0616 CRTC1 MAML2, MECT1, Mam-2, TORC-1, TORC1, WAMTP1 CREB regulated transcription coactivator 1 CREB-regulated transcription coactivator 1|Mastermind-like protein 2|mucoepidermoid carcinoma translocated protein 1|transducer of regulated cAMP response element-binding protein (CREB) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRTC1, check out the CRTC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRTC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TORC1/CRTC1 Antibody Picoband® (A01951-2)
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