Anti-LMTK3 Antibody Picoband™

Product Name

Anti-LMTK3 Antibody Picoband™

View all LMTK3 Antibodies

SKU/Catalog Number

A08432-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LMTK3 Antibody Picoband™ catalog # A08432-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LMTK3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08432-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human LMTK3 recombinant protein (Position: S1317-D1382).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08432-2 is reactive to LMTK3 in Human, Mouse, Rat

Applications

A08432-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

153 kDa

Calculated molecular weight

Background of LMTK3

Lemur tyrosine kinase 3 is a protein that in humans is encoded by the LMTK3 gene. It is mapped to 19q13.33. LMTK3 (lemur tyrosine kinase 3), also known as LMR3 or TYKLM3, is a 1,460 amino acid protein that contains one protein kinase domain. One of several members of the protein kinase superfamily, LMTK3 is expressed at low levels in brain and testis where it catalyzes the ATP-dependent phosphorylation of target proteins, thereby modifying their function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates
Lane 2: human PC-3 whole cell lysates
Lane 3: human U2OS whole cell lysates
Lane 4: mouse thymus tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMTK3 antigen affinity purified polyclonal antibody (Catalog # A08432-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMTK3 at approximately 153KD. The expected band size for LMTK3 is at 153KD.

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Figure 2. IHC analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).
LMTK3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LMTK3 Antibody (A08432-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).
LMTK3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LMTK3 Antibody (A08432-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For LMTK3 (Source: Uniprot.org, NCBI)

Alternative Names

AATYK3; EC 2.7.11.1; lemur tyrosine kinase 3KIAA1883TYKLM3LMR3; serine/threonine-protein kinase LMTK3 LMTK3 LMR3, PPP1R101, TYKLM3 lemur tyrosine kinase 3 serine/threonine-protein kinase LMTK3|protein phosphatase 1, regulatory subunit 101

For more info on LMTK3, check out the LMTK3 Infographic

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In this infographic, you will see the following information for LMTK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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