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Product Info Summary
| SKU: | M01281-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD81 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01281-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD81 Rabbit Monoclonal Antibody catalog # M01281-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD81 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01281-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GAA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD81
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01281-1 is reactive to CD81 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
25809 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01281-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: Human prostate cancer tissue, Human testis cancer tissue, Human Hodgkin's lymphoma tissue, human tonsil tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:2000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:400 dilution.
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Western blot analysis of CD81 expression in Ramos cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD81 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CD81 (Source: Uniprot.org, NCBI)
Gene Name
CD81
Full Name
CD81 antigen
Weight
25809 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD81 antigen;26 kDa cell surface protein TAPA-1;Target of the antiproliferative antibody 1;Tetraspanin-28;Tspan-28;CD81;CD81;TAPA1, TSPAN28; CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 |26 kDa cell surface protein TAPA-1|CD81 (target of antiproliferative 1)|tetraspanin-28|tspan-28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD81, check out the CD81 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD81 Rabbit Monoclonal Antibody (M01281-1)
Hello CJ!
M01281-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cao G,Meng X, Han X,Li J.Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.Biosci Rep.2020 May 29;40(5): BSR20193436.doi:10.1042/BSR20193436.PMID:32400849;PMC
Species: Mouse
M01281-1 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELLS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-CD81 Rabbit Monoclonal Antibody
Question
My question regarding product M01281-1, anti-CD81 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01281-1 anti-CD81 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-17
Question
We have seen staining in mouse brain. Any tips? Is anti-CD81 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
From what I have seen in literature brain does express CD81. From what I have seen in Uniprot.org, CD81 is expressed in myometrium, brain, among other tissues. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-06
Question
What are the contents of the Anti-CD81 Rabbit Monoclonal Antibody M01281-1?
Verified customer
Asked: 2019-10-28
Answer
The contents of the Anti-CD81 Rabbit Monoclonal Antibody M01281-1 are Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Boster Scientific Support
Answered: 2019-10-28
Question
What is the immunogen information for M01281-1 (sequence, any epitope information)?
Verified customer
Asked: 2019-10-24
Answer
The immunogen sequence for the Anti-CD81 Rabbit Monoclonal Antibody (M01281-1) is IISNLFKEDCHQKID. We do not have any other epitope information about the immunogen.
Boster Scientific Support
Answered: 2019-10-25
Question
Is there a BSA free version of anti-CD81 Rabbit Monoclonal antibody M01281-1 available?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD81 Rabbit Monoclonal antibody M01281-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-27
Question
Our lab want to know about to test anti-CD81 Rabbit Monoclonal antibody M01281-1 on mouse brain for research purposes, then I may be interested in using anti-CD81 Rabbit Monoclonal antibody M01281-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The products we sell, including anti-CD81 Rabbit Monoclonal antibody M01281-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-02
Question
I see that the anti-CD81 Rabbit Monoclonal antibody M01281-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-11
Question
Please see the WB image, lot number and protocol we used for brain using anti-CD81 Rabbit Monoclonal antibody M01281-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Is this M01281-1 anti-CD81 Rabbit Monoclonal antibody reactive to the isotypes of CD81?
T. Huang
Verified customer
Asked: 2019-05-17
Answer
The immunogen of M01281-1 anti-CD81 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD81 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-17
Question
Does M01281-1 anti-CD81 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
It shows on the product datasheet, M01281-1 anti-CD81 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CD81 Rabbit Monoclonal antibody M01281-1. Let me know if you need anything else.
S. Kulkarni
Verified customer
Asked: 2018-10-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-23
Question
Does anti-CD81 Rabbit Monoclonal antibody M01281-1 work on canine IF with brain?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
Our lab technicians have not tested anti-CD81 Rabbit Monoclonal antibody M01281-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD81 Rabbit Monoclonal antibody M01281-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-14
Question
We ordered your anti-CD81 Rabbit Monoclonal antibody for IHC on myometrium a few years ago. I am using mouse, and We are going to use the antibody for Flow Cytometry next. I am interested in examining myometrium as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
W. Patel
Verified customer
Asked: 2018-03-06
Answer
I have checked the website and datasheets of our anti-CD81 Rabbit Monoclonal antibody and I see that M01281-1 has been tested on mouse in both IHC and Flow Cytometry. Thus M01281-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-06
Question
We are currently using anti-CD81 Rabbit Monoclonal antibody M01281-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
V. Wu
Verified customer
Asked: 2017-05-31
Answer
The anti-CD81 Rabbit Monoclonal antibody (M01281-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-31
Question
Would anti-CD81 Rabbit Monoclonal antibody M01281-1 work for IHC with brain?
R. Huang
Verified customer
Asked: 2017-05-11
Answer
According to the expression profile of brain, CD81 is highly expressed in brain. So, it is likely that anti-CD81 Rabbit Monoclonal antibody M01281-1 will work for IHC with brain.
Boster Scientific Support
Answered: 2017-05-11
Question
Is a blocking peptide available for product anti-CD81 Rabbit Monoclonal antibody (M01281-1)?
E. Mangal
Verified customer
Asked: 2017-02-23
Answer
We do provide the blocking peptide for product anti-CD81 Rabbit Monoclonal antibody (M01281-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-23
Question
My colleagues were satisfied with the WB result of your anti-CD81 Rabbit Monoclonal antibody. However we have seen positive staining in myometrium cell membrane using this antibody. Is that expected? Could you tell me where is CD81 supposed to be expressed?
A. Lewis
Verified customer
Asked: 2013-03-04
Answer
Based on literature, myometrium does express CD81. Generally CD81 expresses in cell membrane. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-03-04
Question
I was wanting to use your anti-CD81 Rabbit Monoclonal antibody for IHC for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
J. Kulkarni
Verified customer
Asked: 2013-02-22
Answer
It shows on the product datasheet, M01281-1 anti-CD81 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-22
