Anti-PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Monoclonal Antibody

Product Name

Anti-PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Monoclonal Antibody

View all PD-1 Antibodies

SKU/Catalog Number

M00178

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Monoclonal Antibody (Catalog # M00178). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00178)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM597

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human PDCD1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00178 is reactive to PDCD1 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

31647 MW

Background of PD-1

PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00178 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For PDCD1 (Source: Uniprot.org, NCBI)

Alternative Names

Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1; PDCD1 CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 programmed cell death 1 programmed cell death protein 1|programmed cell death 1 protein|protein PD-1|systemic lupus erythematosus susceptibility 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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