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Product Info Summary
| SKU: | M00125-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PCNA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00125-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PCNA Rabbit Monoclonal Antibody catalog # M00125-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCNA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00125-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PCNA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00125-1 is reactive to PCNA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated molecular weight
28769 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00125-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:3000-1:10000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50-1:100
FC 1:200-1:500
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human U-937 whole cell, human 293T whole cell, mouse spleen tissue, rat RAW2647 tissue
IHC: human colon tissue
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (M00125-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-937 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: rat RAW264.7 tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified monoclonal antibody (Catalog # M00125-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using PCNA Antibody.
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Figure 3. IF analysis of PCNA using anti-PCNA antibody (M00125-1) and anti-Beta Tubulin antibody (M01857-3).
PCNA was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:100 with rabbit anti-PCNA Antibody (M00125-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
Protein Target Info & Infographic
Gene/Protein Information For PCNA (Source: Uniprot.org, NCBI)
Gene Name
PCNA
Full Name
Proliferating cell nuclear antigen
Weight
28769 MW
Superfamily
PCNA family
Alternative Names
Proliferating cell nuclear antigen;PCNA;Cyclin;PCNA; PCNA ATLD2 proliferating cell nuclear proliferating cell nuclear |DNA polymerase delta auxiliary protein|cyclin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCNA, check out the PCNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCNA Rabbit Monoclonal Antibody (M00125-1)
Hello CJ!
M00125-1 has been cited in 63 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma Q,Gu JT,Wang B,Feng J,Yang L,Kang XW,Duan P,Sun X,Liu PJ,Wang JC.PlGF signaling and macrophage repolarization contribute to the anti-neoplastic effect of metformin.Eur J Pharmacol.2019 Nov 15;863:172696.doi:10.1016/j.ejphar.2019.172696.Epub 2019 Sep 25.PMID:31562866.
Species: Human,Mouse
M00125-1 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA
Yi M,Zhang J,Li A,Niu M,Yan Y,Jiao Y,Luo S,Zhou P,Wu K.The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-β and PD-L1.J Hematol Oncol.2021 Feb 16;14(1):27.doi:10.1186/s13045-021-0104
Species: Human,Mouse
M00125-1 usage in article: APP:IHC, SAMPLE:TUMOR TISSUE, DILUTION:NA
Zhang F,Zhang L,He L,Cao M,Yang Y,Duan X,Shi J,Liu K.A PITX2 splice-site mutation in a family with Axenfeld-Rieger syndrome leads to decreased expression of nuclear PITX2 protein.Int Ophthalmol.2021 Jan 25.doi:10.1007/s10792-021-01704-5.Epub ahead of prin
Species: Human
M00125-1 usage in article: APP:WB, SAMPLE:LYMPHOCYTES, DILUTION:NA
Yiwei Shi,Xiaojiang Qin,Xiaomin Hou et al.Overexpression of Krüppel-Like Factor 4 (KLF4) Inhibits the Proliferation of Pulmonary Arterial Smooth Muscle Cells Through ERK1/2 Signaling Under Hypoxia Environment,18 January 2021,PREPRINT (Version 1) available
Species: Rat
M00125-1 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:200
Nicotinamide Supplementation Attenuates Renal Interstitial Fibrosis via Boosting the Activity of Sirtuins. Xin Zhen,Shaowu Zhang,Feifei Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Jing Nie
Species: Mouse
M00125-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND PTCS, DILUTION:NA
Xie,J.,Liu,W.,Lv,W.,Han,X.,Kong,Q.,Wu,Y.,Liu,X.,Han,Y.,Shi,C.,& Jia,X.(2020).Transmembrane protein 16A/anoctamin 1 inhibitor T16Ainh-A01 reversed monocrotaline-induced rat pulmonary arterial hypertension. Pulmonary Circulation. https://doi.org/10.1177/204
Species: Rat
M00125-1 usage in article: APP:WB, SAMPLE:PA TISSUE, DILUTION:1:250
Guo,Hongrui & Li,Xinyang & Li,Xiaodan & Jiang, Xinyue & Lai,Weiming & Geng,Yi & Ouyang,Ping & He,Ran & Tang,Huaqiao & Xie,Yue & Yang,Guang-You & Gu,Xiaobin.(2020).Immunologic Improvement of Vaccine for Newcastle Disease via Co-Inoculation of Cattle Transf
Species: Broiler
M00125-1 usage in article: APP:IHC, SAMPLE:SPLEEN,THYMUS AND BURSA OF FABRICIUS, DILUTION:1:100
Zhou,W.,Xi,D.,Shi,Y.,Wang,L.,Zhong,H.,Huang,Z. ... He, F.(2021).MicroRNA‑1929‑3p participates in murine cytomegalovirus‑induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation. International Journal of Molecular Medicine,47,71
Species: Mouse
M00125-1 usage in article: APP:WB, SAMPLE:AORTIC TISSUE, DILUTION:1:250
Study of the correlation between the expression of nuclear factor kappa B and proliferation regulatory proteins and chronic superficial gastritis,Hu Hui,Ma Zhijian,Ren Shouzhong,Xie Yiqiang, Vojnosanitetski pregled 2020 OnLine-First,00,SP - 135 EP-135
Species: Rat
M00125-1 usage in article: APP:IHC, SAMPLE:STOMACH TISSUE, DILUTION:NA
Zhang H,Liu S,Cai Z,Dong W,Ye J,Cai Z,Han Z,Liang Y,Zhuo Y,Luo Y,Zhu X,Deng Y,Zhang Y,Liu R,Feng Y, Lai J,Zhou R,Tan H,Zhong W. Down-regulation of ACACA suppresses the malignant progression of Prostate Cancer through inhibiting mitochondrial potential. J
Species: Human
M00125-1 usage in article: APP:IHC, SAMPLE:REGIONAL LYMPH NODE AND PROSTATE TISSUE, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PCNA Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-PCNA Rabbit Monoclonal antibody M00125-1 available?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PCNA Rabbit Monoclonal antibody M00125-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
Is a blocking peptide available for product anti-PCNA Rabbit Monoclonal antibody (M00125-1)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do provide the blocking peptide for product anti-PCNA Rabbit Monoclonal antibody (M00125-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-17
Question
My team were happy with the WB result of your anti-PCNA Rabbit Monoclonal antibody. However we have observed positive staining in placenta nucleus using this antibody. Is that expected? Could you tell me where is PCNA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Based on literature, placenta does express PCNA. Generally PCNA expresses in nucleus. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-10
Question
Will M00125-1 anti-PCNA Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
As indicated on the product datasheet, M00125-1 anti-PCNA Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-27
Question
I was wanting to use your anti-PCNA Rabbit Monoclonal antibody for IF for mouse skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse skeletal muscle identification?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
It shows on the product datasheet, M00125-1 anti-PCNA Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-15
Question
I see that the anti-PCNA Rabbit Monoclonal antibody M00125-1 works with IF, what is the protocol used to produce the result images on the product page?
K. Walker
Verified customer
Asked: 2019-04-24
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-24
Question
Is this M00125-1 anti-PCNA Rabbit Monoclonal antibody reactive to the isotypes of PCNA?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
The immunogen of M00125-1 anti-PCNA Rabbit Monoclonal antibody is A synthesized peptide derived from human PCNA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-23
Question
Does anti-PCNA Rabbit Monoclonal antibody M00125-1 work for IF with skeletal muscle?
D. Jones
Verified customer
Asked: 2017-10-02
Answer
According to the expression profile of skeletal muscle, PCNA is highly expressed in skeletal muscle. So, it is likely that anti-PCNA Rabbit Monoclonal antibody M00125-1 will work for IF with skeletal muscle.
Boster Scientific Support
Answered: 2017-10-02
Question
We ordered your anti-PCNA Rabbit Monoclonal antibody for IHC on skeletal muscle a few months ago. I am using rat, and We are going to use the antibody for IP next. you antibody examining skeletal muscle as well as fetal brain cortex in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
E. Wu
Verified customer
Asked: 2016-09-19
Answer
I have checked the website and datasheets of our anti-PCNA Rabbit Monoclonal antibody and it appears that M00125-1 has been validated on rat in both IHC and IP. Thus M00125-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-09-19
Question
I have a question about product M00125-1, anti-PCNA Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Jones
Verified customer
Asked: 2016-08-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00125-1 anti-PCNA Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-PCNA Rabbit Monoclonal antibody M00125-1. Let me know if you need anything else.
A. Walker
Verified customer
Asked: 2016-02-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-11
Question
We are interested in to test anti-PCNA Rabbit Monoclonal antibody M00125-1 on mouse skeletal muscle for research purposes, then I may be interested in using anti-PCNA Rabbit Monoclonal antibody M00125-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Anderson
Verified customer
Asked: 2015-05-01
Answer
The products we sell, including anti-PCNA Rabbit Monoclonal antibody M00125-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-01
Question
Here is the WB image, lot number and protocol we used for skeletal muscle using anti-PCNA Rabbit Monoclonal antibody M00125-1. Please let me know if you require anything else.
E. Johnson
Verified customer
Asked: 2015-01-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-02
Question
We have seen staining in rat liver. Any tips? Is anti-PCNA Rabbit Monoclonal antibody supposed to stain liver positively?
R. Zhao
Verified customer
Asked: 2014-07-18
Answer
From what I have seen in literature liver does express PCNA. From what I have seen in Uniprot.org, PCNA is expressed in oocyte, skeletal muscle, bone marrow lung, fetal brain cortex, placenta, liver, among other tissues. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-07-18
Question
We are currently using anti-PCNA Rabbit Monoclonal antibody M00125-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
K. Krishna
Verified customer
Asked: 2014-06-19
Answer
The anti-PCNA Rabbit Monoclonal antibody (M00125-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-19
Question
My lab would like using your anti-PCNA Rabbit Monoclonal antibody for detection of dna damage studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
C. Brown
Verified customer
Asked: 2014-04-02
Answer
Thanks for your inquiry. This M00125-1 anti-PCNA Rabbit Monoclonal antibody is validated on hela cells, hepg2 whole cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-04-02
