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Product Info Summary
| SKU: | M00125 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Chicken, Drosophila, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody
SKU/Catalog Number
M00125
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody (Catalog # M00125). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila, Yeast.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00125)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM350
Isotype
IgG2a, kappa
Immunogen
Rat PCNA/protein A fusion protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00125 is reactive to PCNA in Chicken, Drosophila, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
28769 MW
Background of PCNA
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This monoclonal antibody is excellent for multiple applications.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00125 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-PCNA Mouse Monoclonal Antibody (SPM350).
Protein Target Info & Infographic
Gene/Protein Information For PCNA (Source: Uniprot.org, NCBI)
Gene Name
PCNA
Full Name
Proliferating cell nuclear antigen
Weight
28769 MW
Superfamily
PCNA family
Alternative Names
Proliferating cell nuclear antigen;PCNA;Cyclin;PCNA; PCNA ATLD2 proliferating cell nuclear proliferating cell nuclear |DNA polymerase delta auxiliary protein|cyclin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCNA, check out the PCNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody (M00125)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody
Question
I was wanting to use your anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody for IHC for zebrafish fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for zebrafish fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
It shows on the product datasheet, M00125 anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody has been validated for IHC on chicken, drosophila, human, monkey, mouse, pig, rat, yeast, zebrafish tissues. We have an innovator award program that if you test this antibody and show it works in zebrafish fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
Does anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 work for IHC with fetal brain cortex?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
According to the expression profile of fetal brain cortex, PCNA is highly expressed in fetal brain cortex. So, it is likely that anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 will work for IHC with fetal brain cortex.
Boster Scientific Support
Answered: 2020-03-23
Question
Will M00125 anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
As indicated on the product datasheet, M00125 anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-02
Question
Please see the WB image, lot number and protocol we used for fetal brain cortex using anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125. Please let me know if you require anything else.
Z. Miller
Verified customer
Asked: 2020-01-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-23
Question
Is this M00125 anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody reactive to the isotypes of PCNA?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The immunogen of M00125 anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody is Rat PCNA/Protein A fusion protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-22
Question
We have observed staining in monkey liver. Are there any suggestions? Is anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
According to literature liver does express PCNA. According to Uniprot.org, PCNA is expressed in oocyte, skeletal muscle, bone marrow lung, fetal brain cortex, placenta, liver, among other tissues. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-09
Question
Our lab were satisfied with the WB result of your anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody. However we have seen positive staining in skeletal muscle nucleus using this antibody. Is that expected? Could you tell me where is PCNA supposed to be expressed?
B. Gonzalez
Verified customer
Asked: 2019-11-26
Answer
According to literature, skeletal muscle does express PCNA. Generally PCNA expresses in nucleus. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-26
Question
I have a question about product M00125, anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00125 anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody (M00125)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody (M00125). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
We are currently using anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 for chicken tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says chicken, drosophila, human, monkey, mouse, pig, rat, yeast, zebrafish. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
The anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody (M00125) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-14
Question
Do you have a BSA free version of anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 available?
N. Parker
Verified customer
Asked: 2016-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-08-01
Question
I am interested in to test anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 on zebrafish fetal brain cortex for research purposes, then I may be interested in using anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Bhatt
Verified customer
Asked: 2016-03-18
Answer
The products we sell, including anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-18
Question
I see that the anti-PCNA (Proliferating Cell Nuclear antigen) (G1- & S-phase Marker) Monoclonal antibody M00125 works with IHC, what is the protocol used to produce the result images on the product page?
C. Patel
Verified customer
Asked: 2016-01-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-15
