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Product Info Summary
| SKU: | EK1524 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1524
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human WIF1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human WIF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1524)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G29-W379
Sensitivity
<10 pg/ml
Assay Range
23.4 pg/ml - 1500 pg/ml
Standard Dilution Instructions
See datasheet of EK1524 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1524 is reactive to WIF1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1524 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of WIF-1
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1524-CAP | Anti-Human WIF1 Pre-coated 96-well strip microplate | 1 |
| EK1524-ST | Human WIF1 Standard | 2 vials, 10 ng/tube |
| EK1524-DA | Human WIF1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 23.4 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.039 | 0.442 | 0.264 | 0.442 | 0.732 | 1.229 | 1.827 | 2.127 |
Data Example Images
Click image to see more details
Human WIF1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 43 | 210 | 939 | 40 | 222 | 983 |
| Standard deviation | 2.06 | 11.76 | 46.95 | 2.64 | 15%8 | 67.82 |
| CV (%) | 4.8% | 5.6% | 5% | 6.6% | 7.2% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of WIF-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 43 | 39 | 39 | 39 | 40 | 1.73 | 4.3% |
| Sample 2 | 210 | 211 | 236 | 217 | 218 | 10.45 | 4.7% |
| Sample 3 | 939 | 920 | 839 | 864 | 890 | 40.54 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For WIF1 (Source: Uniprot.Org, NCBI)
Gene Name
WIF1
Full Name
Wnt inhibitory factor 1
Weight
41528 MW
Alternative Names
WIF1; WIF-1; WNT inhibitory factor 1 WIF1 WIF-1 WNT inhibitory factor 1 wnt inhibitory factor 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on WIF1, check out the WIF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for WIF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine® (EK1524)
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Customer Q&As
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7 Customer Q&As for Human WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
C. Mehta
Verified customer
Asked: 2020-09-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-09-25
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-05-11
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring WIF-1 using the EK1524 Human WIF-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-02-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-12-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-12-08
Question
Q: is it okay to use citrate plasma as samples in Human WIF-1 Picokine® ELISA Kit (Catalog # EK1524)?
T. Evans
Verified customer
Asked: 2018-03-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of WIF-1 causing degradation of its protein structure. WIF-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the WIF-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-03-28
Question
Q: which procedure should I follow in order to thaw whole blood sample for WIF-1 ELISA after freezing?
M. Thompson
Verified customer
Asked: 2016-12-25
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test WIF-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-12-25
Question
Q: how do I analyze ELISA data? I measured WIF-1 level in plasma.
B. Carter
Verified customer
Asked: 2016-12-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-12-14
