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Product Info Summary
| SKU: | MA1083 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-PCNA Antibody (Monoclonal, PC 10)
SKU/Catalog Number
MA1083
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCNA Antibody (Monoclonal, PC 10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1083)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
PC 10
Isotype
Mouse IgG2a
Immunogen
Protein A fusion protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1083 is reactive to Pcna in Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
28749 MW
Background of PCNA
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1083 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat
Positive Control
WB: human Caco-2 whole cell, human MDA-MB-231 whole cell, human Jurkat whole cell, human HT1080 whole cell
IHC: human Rectal cancer tissue
ICC: human HELA Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (MA1083).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human MDA-MB-231 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HT1080 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCNA antigen affinity purified monoclonal antibody (Catalog # MA1083) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCNA at approximately 35KD. The expected band size for PCNA is at 29KD.
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Figure 2. IHC analysis of PCNA using anti-PCNA antibody (MA1083).
PCNA was detected in paraffin-embedded section of human Rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of PCNA using anti-PCNA antibody (MA1083).
PCNA was detected in immunocytochemical section of human HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Pcna (Source: Uniprot.org, NCBI)
Gene Name
Pcna
Full Name
Proliferating cell nuclear antigen
Weight
28749 MW
Superfamily
PCNA family
Alternative Names
Proliferating cell nuclear antigen;PCNA;Cyclin;Pcna; PCNA ATLD2 proliferating cell nuclear proliferating cell nuclear |DNA polymerase delta auxiliary protein|cyclin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pcna, check out the Pcna Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pcna: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCNA Antibody (Monoclonal, PC 10) (MA1083)
Hello CJ!
MA1083 has been cited in 131 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Evaluation of TUNEL, PCNA and SOX2 Expressions in Lens Epithelial Cells of Cataract Patients with Pseudoexfoliation Syndrome
Ma Q,Gu JT,Wang B,Feng J,Yang L,Kang XW,Duan P,Sun X,Liu PJ,Wang JC.PlGF signaling and macrophage repolarization contribute to the anti-neoplastic effect of metformin.Eur J Pharmacol.2019 Nov 15;863:172696.doi:10.1016/j.ejphar.2019.172696.Epub 2019 Sep 25.PMID:31562866.
Species: Human,Mouse
MA1083 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA
Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
Species: Rat
MA1083 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
Xiong W,Wu L,Tang R,Zhang Q,Guo Q,Song S.Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis.Cancer Manag Res.2021;13:4359-4371https://doi. org/10.2147/CMAR.S302084
Species: Human,Mouse
MA1083 usage in article: APP:WB, SAMPLE:A431 CELL AND SCL-1 CELL, DILUTION:1:2000
Tan PC,Chao PC,Cheng C,Chen CH,Huang RL,Zhou SB,Xie Y,Li QF.A randomized, controlled clinical trial of autologous stromal vascular fraction cells transplantation to promote mechanical stretch-induced skin regeneration.Stem Cell Res Ther.2021 Apr 15;12(1):243.doi:10.1186/s13287-021-02318-5.PMID:33858504;PMCID:P MC8048343.
Species: Human
MA1083 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:100
Fan R,Wang H,Zhang L,Ma T,Tian Y,Li H.Nanocrystallized Oleanolic Acid Better Inhibits Proliferation, Migration and Invasion in Intracranial Glioma via Caspase-3 Pathway.J Cancer.2020 Jan 29;11(7):1949-1958.doi:10.7150/jca. 38847.PMID:32194806;PMCID:PMC7052863.
Species: Human,Mouse
MA1083 usage in article: APP:IHC, SAMPLE:GLIOMA TISSUE, DILUTION:NA
Yi M,Zhang J,Li A,Niu M,Yan Y,Jiao Y,Luo S,Zhou P,Wu K.The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-β and PD-L1.J Hematol Oncol.2021 Feb 16;14(1):27.doi:10.1186/s13045-021-0104
Species: Human,Mouse
Zhang F,Zhang L,He L,Cao M,Yang Y,Duan X,Shi J,Liu K.A PITX2 splice-site mutation in a family with Axenfeld-Rieger syndrome leads to decreased expression of nuclear PITX2 protein.Int Ophthalmol.2021 Jan 25.doi:10.1007/s10792-021-01704-5.Epub ahead of prin
Species: Human
MA1083 usage in article: APP:WB, SAMPLE:LYMPHOCYTES, DILUTION:NA
Yiwei Shi,Xiaojiang Qin,Xiaomin Hou et al.Overexpression of Krüppel-Like Factor 4 (KLF4) Inhibits the Proliferation of Pulmonary Arterial Smooth Muscle Cells Through ERK1/2 Signaling Under Hypoxia Environment,18 January 2021,PREPRINT (Version 1) available
Species: Rat
MA1083 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:200
Nicotinamide Supplementation Attenuates Renal Interstitial Fibrosis via Boosting the Activity of Sirtuins. Xin Zhen,Shaowu Zhang,Feifei Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Jing Nie
Species: Mouse
MA1083 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE AND PTCS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-PCNA Antibody (Monoclonal, PC 10)
Question
Is this MA1083 anti-PCNA antibody (Monoclonal, PC 10) reactive to the isotypes of PCNA?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The immunogen of MA1083 anti-PCNA antibody (Monoclonal, PC 10) is Protein A fusion protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-03
Question
We have seen staining in rat bone marrow lung. Are there any suggestions? Is anti-PCNA antibody (Monoclonal, PC 10) supposed to stain bone marrow lung positively?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
From literature bone marrow lung does express PCNA. From Uniprot.org, PCNA is expressed in oocyte, skeletal muscle, bone marrow lung, fetal brain cortex, placenta, liver, among other tissues. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-16
Question
Does anti-PCNA antibody (Monoclonal, PC 10) MA1083 work for WB with oocyte?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
According to the expression profile of oocyte, PCNA is highly expressed in oocyte. So, it is likely that anti-PCNA antibody (Monoclonal, PC 10) MA1083 will work for WB with oocyte.
Boster Scientific Support
Answered: 2019-12-11
Question
Please see the WB image, lot number and protocol we used for oocyte using anti-PCNA antibody (Monoclonal, PC 10) MA1083. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-16
Question
I am looking for to test anti-PCNA antibody (Monoclonal, PC 10) MA1083 on human oocyte for research purposes, then I may be interested in using anti-PCNA antibody (Monoclonal, PC 10) MA1083 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-PCNA antibody (Monoclonal, PC 10) MA1083, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
I was wanting to use your anti-PCNA antibody (Monoclonal, PC 10) for WB for human oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human oocyte identification?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
As indicated on the product datasheet, MA1083 anti-PCNA antibody (Monoclonal, PC 10) has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
Do you have a BSA free version of anti-PCNA antibody (Monoclonal, PC 10) MA1083 available?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PCNA antibody (Monoclonal, PC 10) MA1083 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use using your anti-PCNA antibody (Monoclonal, PC 10) for liver regeneration studies. Has this antibody been tested with western blotting on human hela? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you for your inquiry. This MA1083 anti-PCNA antibody (Monoclonal, PC 10) is validated on human hela, jurkat whole cell lysates. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-21
Question
Is a blocking peptide available for product anti-PCNA antibody (Monoclonal, PC 10) (MA1083)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
We do provide the blocking peptide for product anti-PCNA antibody (Monoclonal, PC 10) (MA1083). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-21
Question
Would anti-PCNA antibody (Monoclonal, PC 10) MA1083 work on primate IHC with skeletal muscle?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
Our lab technicians have not tested anti-PCNA antibody (Monoclonal, PC 10) MA1083 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PCNA antibody (Monoclonal, PC 10) MA1083 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skeletal muscle in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-19
Question
I see that the anti-PCNA antibody (Monoclonal, PC 10) MA1083 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-10
Question
We are currently using anti-PCNA antibody (Monoclonal, PC 10) MA1083 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
The anti-PCNA antibody (Monoclonal, PC 10) (MA1083) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-26
Question
My team were satisfied with the WB result of your anti-PCNA antibody (Monoclonal, PC 10). However we have been able to see positive staining in skeletal muscle nucleus using this antibody. Is that expected? Could you tell me where is PCNA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
According to literature, skeletal muscle does express PCNA. Generally PCNA expresses in nucleus. Regarding which tissues have PCNA expression, here are a few articles citing expression in various tissues:
Bone marrow, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 9305916
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 12522211
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-05-12
Question
My question regarding product MA1083, anti-PCNA antibody (Monoclonal, PC 10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Banerjee
Verified customer
Asked: 2017-01-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1083 anti-PCNA antibody (Monoclonal, PC 10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-PCNA antibody (Monoclonal, PC 10) MA1083. Let me know if you need anything else.
K. Moore
Verified customer
Asked: 2016-05-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-09
Question
We purchased anti-PCNA antibody (Monoclonal, PC 10) for IHC on skeletal muscle a few years ago. I am using mouse, and We intend to use the antibody for ICC next. My question regards examining skeletal muscle as well as oocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
G. Huang
Verified customer
Asked: 2014-08-29
Answer
I looked at the website and datasheets of our anti-PCNA antibody (Monoclonal, PC 10) and I see that MA1083 has been tested on mouse in both IHC and ICC. Thus MA1083 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-08-29
Question
Will MA1083 anti-PCNA antibody (Monoclonal, PC 10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Singh
Verified customer
Asked: 2013-03-04
Answer
It shows on the product datasheet, MA1083 anti-PCNA antibody (Monoclonal, PC 10) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-03-04
