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Product Info Summary
| SKU: | M01263-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-Beta-Actin Antibody
View all beta-Actin Antibodies
SKU/Catalog Number
M01263-3
Size
80 µl
Description
Boster Bio Anti-Beta-Actin Antibody (Catalog # M01263-3). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Beta-Actin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-3)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
137CT26.1.1
Isotype
IgG1,Igk
Immunogen
This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01263-3 is reactive to ACTB in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
41737 Da
Background of beta-Actin
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01263-3 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF: 1:10-1:50
IHC-P: 1:25
FC: 1:25
IHC: 1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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All lanes : Anti-ACTB Antibody at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: C2C12 whole cell lysate
Lane 3: C6 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: MCF-7 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-ACTB Antibody in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35g/lane). ACTB (arrow) was detected using the purified Mab.
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All lanes : Anti-ACTB Antibody at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: NIH-3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
M01263-3 staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded H.skeletal muscle section using Beta-Actin Antibody. M01263-3 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody. M01263-3 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41737 Da
Superfamily
actin family
Alternative Names
Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beta-Actin Antibody (M01263-3)
Hello CJ!
M01263-3 has been cited in 64 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu ZZ,Han ZD,Liang YK,Chen JX,Wan S,Zhuo YJ,Cai ZD,Deng YL,Lin ZY,Mo RJ,He HC,Zhong WD.TRIB1 induces macrophages to M2 phenotype by inhibiting IKB-zeta in prostate cancer.Cell Signal.2019 Jul;59:152-162.doi:10.1016/j.cellsig.2019.03.017.Epub 2019 Mar 26.PMID:30926388.
Species: Human,Mouse
M01263-3 usage in article: APP:WB, SAMPLE:PC3 CELL,DU145 CELL AND THP-1 CELL, DILUTION:1:3000
Ma Y,Chai H,Ding Q,Qian Q,Yan Z,Ding B,Dou X,Li S.Hepatic SIRT3 Upregulation in Response to Chronic Alcohol Consumption Contributes to Alcoholic Liver Disease in Mice.Front Physiol.2019 Aug 13;10:1042.doi:10.3389/fphys.2019.01042.PMID:31474877;PMCID:PMC6707764.
Species: Human,Mouse
M01263-3 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Liu J,Yang C,Yang J,Song X,Han W,Xie M,Cheng L,Xie L,Chen H,Jiang L.Effects of early postnatal exposure to fine particulate matter on emotional and cognitive development and structural synaptic plasticity in immature and mature rats.Brain Behav.2019 Dec;9(12):e01453.doi:10.1002/brb3.1453.Epub 2019 Nov 11.PMID:31709780; PMCID:PMC6908876.
Species: Rat
M01263-3 usage in article: APP:WB, SAMPLE: HIPPOCAMPI, DILUTION:1:500
Zhang T,Ma L,Wu P,Li W,Li T,Gu R,Dan X,Li Z,Fan X,Xiao Z.Gallic acid has anticancer activity and enhances the anticancer effects of cisplatin in non‑small cell lung cancer A549 cells via the JAK/STAT3 signaling pathway.Oncol Rep.2019 Mar;41(3):1779-1788.doi:10.3892/or.2019.6976.Epub 2019 Jan 22.PMID:30747218.
Species: Human
M01263-3 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:1000
Yao X,Jing X,Guo J,Sun K,Deng Y,Zhang Y,Guo F,Ye Y.Icariin Protects Bone Marrow Mesenchymal Stem Cells Against Iron Overload Induced Dysfunction Through Mitochondrial Fusion and Fission, PI3K/AKT/mTOR and MAPK Pathways.Front Pharmacol.2019 Feb 28;10:163. doi:10.3389/fphar.2019.00163.PMID:30873034;PMCID:PMC6403125.
Species: Rat
M01263-3 usage in article: APP: WB, SAMPLE:BMSCS, DILUTION:NA
Liu F,Zhang YY,Song N,Lin J,Liu MK,Huang CL,Zhou C,Wang H,Wang M,Shen JF.GABAB receptor activation attenuates inflammatory orofacial pain by modulating interleukin-1β in satellite glial cells: Role of NF-κB and MAPK signaling pathways.Brain Res Bull.2019 Jul;149:240-250.doi:10.1016/j.brainresbull.2019.04.018.Epub 2019 Apr 26.PMID:31034945.
Species: Rat
M01263-3 usage in article: APP:WB, SAMPLE:SGCS, DILUTION:1:200
Li Z,Yulei J,Yaqing J,Jinmin Z,Xinyong L,Jing G,Min L.Protective effects of tetramethylpyrazine analogue Z-11 on cerebral ischemia reperfusion injury.Eur J Pharmacol.2019 Feb 5;844:156-164.doi:10.1016/j.ejphar.2018.11.031.Epub 2018 Nov 29.PMID:30502344.
Species: Mouse,Rat
M01263-3 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA
Yiping Lin,Jingchan Yao,Meiling Wu,Xiaoqian Ying,Mingxing Ding,Yanli Wei,Xiaoyan Fu,Wei Feng,Yunguang Wang,"Tetrandrine Ameliorates Airway Remodeling of Chronic Asthma by Interfering TGF-β1/Nrf-2/HO-1 Signaling Pathway-Mediated Oxidative Stress",Canadian Respiratory Journal,vol.2019,Article ID 7930396,12 pages,2019.https://doi.org/10.1155/2019/7930396
Species: Rat
M01263-3 usage in article: APP:WB, SAMPLE:ASMCS, DILUTION:NA
Li R,Liu Y,Shi J,Yu Y,Lu H,Yu L,Liu Y,Zhang F.Diosgenin regulates cholesterol metabolism in hypercholesterolemic rats by inhibiting NPC1L1 and enhancing ABCG5 and ABCG8.Biochim Biophys Acta Mol Cell Biol Lipids.2019 Aug;1864(8):1124-1133.doi:10.1016/j.bbalip.2019.04.010.Epub 2019 May 1.PMID:31054325.
Species: Rat
M01263-3 usage in article: APP:WB, SAMPLE:INTESTINE AND LIVER TISSUE, DILUTION:1:5000
Wang X,Wu L,Zhang Q,Li L,Xie Y,Wan X,Wu H,Xiang Y.Methyl 3,4-dihydroxybenzoate protects against d-galN/LPS-induced acute liver injury by inhibiting inflammation and apoptosis in mice.J Pharm Pharmacol.2019 Jul;71(7):1082-1088.doi:10.1111/jphp.13091.Epub 2019 Apr 29.PMID:31032922.
Species: Mouse
M01263-3 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:4000
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