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Product Info Summary
| SKU: | PB9768 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PAX6 Antibody Picoband®
SKU/Catalog Number
PB9768
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAX6 Antibody Picoband® catalog # PB9768. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9768)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PAX6, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9768 is reactive to PAX6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
46683 MW
Background of PAX6
Paired box protein Pax-6, also known as aniridia type II protein (AN2) or oculorhombin, is a protein that in humans is encoded by the PAX6 gene. This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. In addition, this gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9768 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, U87 Whole Cell, HELA Whole Cell
IHC: mouse pancreas tissue, rat pancreas tissue, human pancreatic cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PAX6 using anti-PAX6 antibody (PB9768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: U87 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 antigen affinity purified polyclonal antibody (Catalog # PB9768) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX6 at approximately 47 kDa. The expected band size for PAX6 is at 47 kDa.
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Figure 2. IHC analysis of PAX6 using anti-PAX6 antibody (PB9768).
PAX6 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAX6 Antibody (PB9768) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of PAX6 using anti-PAX6 antibody (PB9768).
PAX6 was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAX6 Antibody (PB9768) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of PAX6 using anti-PAX6 antibody (PB9768).
PAX6 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAX6 Antibody (PB9768) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PAX6 (Source: Uniprot.org, NCBI)
Gene Name
PAX6
Full Name
Paired box protein Pax-6
Weight
46683 MW
Superfamily
paired homeobox family
Alternative Names
Paired box protein Pax-6;Aniridia type II protein;Oculorhombin;PAX6;AN2; PAX6 AN, AN1, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR paired box 6 paired box protein Pax-6|Aniridia 1|Aniridia 2|aniridia type II protein|oculorhombin|paired box homeotic gene-6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX6, check out the PAX6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX6 Antibody Picoband® (PB9768)
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No publications found for PB9768
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Customer Q&As
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16 Customer Q&As for Anti-PAX6 Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-PAX6 antibody PB9768. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PAX6 antibody PB9768. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
I see that the anti-PAX6 antibody PB9768 works with IHC, what is the protocol used to produce the result images on the product page?
D. Williams
Verified customer
Asked: 2020-04-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-07
Question
Our lab were satisfied with the WB result of your anti-PAX6 antibody. However we have seen positive staining in fetal eye nucleus. using this antibody. Is that expected? Could you tell me where is PAX6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
According to literature, fetal eye does express PAX6. Generally PAX6 expresses in nucleus. Regarding which tissues have PAX6 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Fetal eye, Pubmed ID: 1684738
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-09
Question
I have a question about product PB9768, anti-PAX6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9768 anti-PAX6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-04
Question
I would like using your anti-PAX6 antibody for and inactivation of glucose-dependent insulinotropic polypeptide (gip) studies. Has this antibody been tested with western blotting on mouse pancreas tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
Thanks for your inquiry. This PB9768 anti-PAX6 antibody is validated on rat pancreas tissue, brain tissue, tissue lysate, mouse pancreas tissue, u87 whole cell lysate, hela whole cell lysate, pancreatic cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-16
Question
Does PB9768 anti-PAX6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
As indicated on the product datasheet, PB9768 anti-PAX6 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
Is there a BSA free version of anti-PAX6 antibody PB9768 available?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAX6 antibody PB9768 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-04
Question
Will anti-PAX6 antibody PB9768 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
According to the expression profile of cervix carcinoma, PAX6 is highly expressed in cervix carcinoma. So, it is likely that anti-PAX6 antibody PB9768 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2019-01-16
Question
Our lab used your anti-PAX6 antibody for IHC on fetal eye a few years ago. I am using human, and We want to use the antibody for WB next. We are interested in examining fetal eye as well as lung in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
I took a look at the website and datasheets of our anti-PAX6 antibody and I see that PB9768 has been tested on human in both IHC and WB. Thus PB9768 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-17
Question
Is a blocking peptide available for product anti-PAX6 antibody (PB9768)?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
We do provide the blocking peptide for product anti-PAX6 antibody (PB9768). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-07
Question
We have seen staining in human palpebral conjunctiva. Are there any suggestions? Is anti-PAX6 antibody supposed to stain palpebral conjunctiva positively?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
From literature palpebral conjunctiva does express PAX6. From Uniprot.org, PAX6 is expressed in palpebral conjunctiva, fetal eye, cerebellum, lung, cervix carcinoma, among other tissues. Regarding which tissues have PAX6 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Fetal eye, Pubmed ID: 1684738
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-17
Question
Is this PB9768 anti-PAX6 antibody reactive to the isotypes of PAX6?
W. Jones
Verified customer
Asked: 2016-02-19
Answer
The immunogen of PB9768 anti-PAX6 antibody is A synthetic peptide corresponding to a sequence in the middle region of human PAX6 (195-233aa EDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTH), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-02-19
Question
I was wanting to use your anti-PAX6 antibody for IHC for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
R. Huang
Verified customer
Asked: 2015-09-11
Answer
You can see on the product datasheet, PB9768 anti-PAX6 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-11
Question
I am interested in to test anti-PAX6 antibody PB9768 on rat cervix carcinoma for research purposes, then I may be interested in using anti-PAX6 antibody PB9768 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Krishna
Verified customer
Asked: 2014-04-01
Answer
The products we sell, including anti-PAX6 antibody PB9768, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-04-01
Question
We are currently using anti-PAX6 antibody PB9768 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
D. Evans
Verified customer
Asked: 2013-03-29
Answer
The anti-PAX6 antibody (PB9768) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-29
