Anti-PAX6 (Stem Cell Marker) Monoclonal Antibody

Product Name

Anti-PAX6 (Stem Cell Marker) Monoclonal Antibody

View all PAX6 Antibodies

SKU/Catalog Number

M00273-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-PAX6 (Stem Cell Marker) Monoclonal Antibody (Catalog # M00273-1). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human, Mouse, Rat, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-PAX6 (Stem Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00273-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PAX6/498

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (N-terminus; aa 1-300) of human PAX6 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00273-1 is reactive to PAX6 in Chicken, Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

46683 MW

Background of PAX6

Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00273-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For PAX6 (Source: Uniprot.org, NCBI)

Alternative Names

Paired box protein Pax-6;Aniridia type II protein;Oculorhombin;PAX6;AN2; PAX6 AN, AN1, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR paired box 6 paired box protein Pax-6|Aniridia 1|Aniridia 2|aniridia type II protein|oculorhombin|paired box homeotic gene-6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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