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Product Info Summary
| SKU: | M00273-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-PAX6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00273-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAX6 Rabbit Monoclonal Antibody catalog # M00273-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00273-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HEB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAX6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00273-2 is reactive to PAX6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
46683 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00273-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
FC 1:500
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of PAX6 expression in HeLa cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For PAX6 (Source: Uniprot.org, NCBI)
Gene Name
PAX6
Full Name
Paired box protein Pax-6
Weight
46683 MW
Superfamily
paired homeobox family
Alternative Names
Paired box protein Pax-6;Aniridia type II protein;Oculorhombin;PAX6;AN2; PAX6 AN, AN1, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR paired box 6 paired box protein Pax-6|Aniridia 1|Aniridia 2|aniridia type II protein|oculorhombin|paired box homeotic gene-6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX6, check out the PAX6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX6 Rabbit Monoclonal Antibody (M00273-2)
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No publications found for M00273-2
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Customer Q&As
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16 Customer Q&As for Anti-PAX6 Rabbit Monoclonal Antibody
Question
We have seen staining in rat cervix carcinoma. What should we do? Is anti-PAX6 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to literature cervix carcinoma does express PAX6. According to Uniprot.org, PAX6 is expressed in palpebral conjunctiva, fetal eye, cerebellum, lung, cervix carcinoma, among other tissues. Regarding which tissues have PAX6 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Fetal eye, Pubmed ID: 1684738
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-16
Question
My question regards using your anti-PAX6 Rabbit Monoclonal antibody for commitment of neuronal cell to specific neuron type in forebrain studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We appreciate your inquiry. This M00273-2 anti-PAX6 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-08
Question
Could you provide the epitope information of M00273-2?
Verified customer
Asked: 2019-12-03
Answer
The immunogen sequence of the Anti-PAX6 Rabbit Monoclonal Antibody (M00273-2) is 417-422aa PGSEPDMSQYWPRLQ.
Boster Scientific Support
Answered: 2019-12-05
Question
I have a question about product M00273-2, anti-PAX6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00273-2 anti-PAX6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
Is there a BSA free version of anti-PAX6 Rabbit Monoclonal antibody M00273-2 available?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PAX6 Rabbit Monoclonal antibody M00273-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-29
Question
We are interested in to test anti-PAX6 Rabbit Monoclonal antibody M00273-2 on human palpebral conjunctiva for research purposes, then I may be interested in using anti-PAX6 Rabbit Monoclonal antibody M00273-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The products we sell, including anti-PAX6 Rabbit Monoclonal antibody M00273-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-24
Question
I was wanting to use your anti-PAX6 Rabbit Monoclonal antibody for WB for human palpebral conjunctiva on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human palpebral conjunctiva identification?
O. Moore
Verified customer
Asked: 2019-09-13
Answer
As indicated on the product datasheet, M00273-2 anti-PAX6 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human palpebral conjunctiva in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Is this M00273-2 anti-PAX6 Rabbit Monoclonal antibody reactive to the isotypes of PAX6?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The immunogen of M00273-2 anti-PAX6 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAX6 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-29
Question
Is a blocking peptide available for product anti-PAX6 Rabbit Monoclonal antibody (M00273-2)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
We do provide the blocking peptide for product anti-PAX6 Rabbit Monoclonal antibody (M00273-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-25
Question
I see that the anti-PAX6 Rabbit Monoclonal antibody M00273-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-03
Question
Does anti-PAX6 Rabbit Monoclonal antibody M00273-2 work for WB with palpebral conjunctiva?
V. Parker
Verified customer
Asked: 2019-05-30
Answer
According to the expression profile of palpebral conjunctiva, PAX6 is highly expressed in palpebral conjunctiva. So, it is likely that anti-PAX6 Rabbit Monoclonal antibody M00273-2 will work for WB with palpebral conjunctiva.
Boster Scientific Support
Answered: 2019-05-30
Question
Our team were satisfied with the WB result of your anti-PAX6 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is PAX6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
According to literature, cervix carcinoma does express PAX6. Generally PAX6 expresses in nucleus. Regarding which tissues have PAX6 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Fetal eye, Pubmed ID: 1684738
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-06-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for palpebral conjunctiva using anti-PAX6 Rabbit Monoclonal antibody M00273-2. Let me know if you need anything else.
D. Li
Verified customer
Asked: 2018-05-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-04
Question
We are currently using anti-PAX6 Rabbit Monoclonal antibody M00273-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
D. Li
Verified customer
Asked: 2016-07-19
Answer
The anti-PAX6 Rabbit Monoclonal antibody (M00273-2) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-19
Question
Will M00273-2 anti-PAX6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Williams
Verified customer
Asked: 2015-11-05
Answer
You can see on the product datasheet, M00273-2 anti-PAX6 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-11-05
Question
Here is the WB image, lot number and protocol we used for palpebral conjunctiva using anti-PAX6 Rabbit Monoclonal antibody M00273-2. Please let me know if you require anything else.
P. Jackson
Verified customer
Asked: 2015-06-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-29
