Paired box protein Pax-6 Antibodies

Paired box protein Pax-6 (PAX6)

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity).

Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity).

Gene Information

Human
Gene Name:	PAX6
Uniprot:	P26367
Entrez:	5080

Family

Belongs to:
paired homeobox family

Alternative Names

keratitis); MGC17209; Oculorhombin; paired box 6; paired box protein Pax-6; Pax6

Molecular Weight

Mass (kDA):
46.683 kDA

Genomic Context

Human
Location:	11p13
Sequence:	11; NC_000011.10 (31784792..31817961, complement)

Tissue Specificity

Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.

Subcellular Localizations

Nucleus.

Diseases

• Aniridia
• Nervousness
• Corneal Diseases
• Eye Abnormalities
• Neoplasms
• Hypoplasia
• Microphthalmos
• Haploinsufficiency
• Cataract
• Tissue Adhesions
• Diabetes Mellitus
• Malignant Neoplasms

• Congenital Abnormality
• Glaucoma
• Nystagmus
• Nephroblastoma
• Congenital Ocular Coloboma (disorder)
• Disorder Of Eye
• Wagr Syndrome

Interacting Proteins

• Dynll1
• HOMER3
• Sumo1

Pathways

• Eye Development
• Neurogenesis
• Cell Proliferation
• Cell Differentiation
• Regeneration
• Cell Cycle
• Localization
• Brain Development
• Secretion
• Transdifferentiation
• Cell Migration
• Methylation
• Regionalization

• Cell Death
• Cell Development
• System Development
• Eye Morphogenesis
• Pathogenesis
• Cell Adhesion
• Pancreas Development

PTMs

• Methylation
• Phosphorylation
• Cleavage
• Acetylation
• Ribosylation

• Sumoylation
• Demethylation
• Deacetylation
• Glycosylation
• Oxidation

Research Areas

• Cellular Markers
• Diabetes Research
• Neuroscience
• Sensory Systems
• Stem Cell Markers

• Transcription Factors and Regulators
• Vision

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PAX6

Best Uses Of The PAX6 Marker From Boster Bio

You have come to the right spot if you are looking for a PAX6 marker of high quality. This article will explain the benefits of the PAX6 Polyclonal Antibody, the Picogram Sensitivity ELISA Kit, as well as how to customize the kit for your specific needs. If you are looking for custom services, we recommend ordering a kit.

Anti-PAX6 antibody

Boster BIO's Anti-PAX6 antibody reacts to Human PAX6 protein. This antibody is available as a Monoclonal Antibody and is validated for use in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry. It can keep at 4°C for a month. This antibody is prepared using rabbits immunized in synthetic peptide.

This monoclonal antibody was approved for use in immunofluorescence or Western blot techniques. It was tested using Rat, Mouse,, and Human tissues. Antibody can keep at -20C for at least one year. Repeated freezing or thawing can cause it to lose its potency. Boster Bio's Anti-PAX6 antibody can be ordered through a reputable business to ensure the highest quality products.

Pax6 plays an important role in the development of the central nerve system and sensory organs. It regulates gene transcription by using two DNA-binding domains. The gene's PD domain and HD domain function independently to regulate various aspects of the gene’s functions. They regulate the formation, development, and maintenance of the retina and lens during eye development. These properties of Pax6 allow it to function in a variety of experiments and to be used as a research tool.

Boster Biologicals offers the Anti-PAX6 antibody in a variety formats. The company makes ELISA kits and research antibodies for a variety applications. They provide a picogram level sensitivity and have been validated in IHC and WB. The BosterBio immunological agents are available through tebubio. Boster Bio's anti-PAX6 antibody is compatible with IHC, ELISA and Flow Cymetry.

IHC-optimized, polyclonal antibody

Boster Bio's IHC-optimized PAX6 monoclonal anti-rabbit monoclonal antibody reacts to Human, Mouse and Rat. Boster validates all its antibodies using multiple methods such as WB and IHC. The PAX6 marker is a well-known candidate for PAX6 immunohistochemistry, but it is also useful for other applications.

This study involved NSC-6 immunolabeling using hNPCs derived mainly from wild-type C57/BL6 mouse. The antibody was used for labelling many PAX6+ NSCs. However, it did not label any immature DCX+ neurons or S100b+ astrocytes in the 55d organoid.

To validate the antigen, the mouse antibodies were raised against cultures of mouse embryonic neurospheres containing neural stem cells and progenitor cells. The NSC-6 Ab showed the strongest staining on NPCs. This corresponded closely to BASP1, an expression previously unknown in NPCs. The NSC-6 Ab was used for the first time in this study to identify NSCs.

After primaries were diluted using blocking buffer, the sections of the tissue were stained with biotinylated antibodies in PBS. The secondary antibodies were then incubated at room temperature for two hours. The samples were then washed with 0.1% Triton X100 – PBS and mounted by ProLong Diamond Antifade Mountant.

For the detection of NSC-6, the PAX6-specific antibody from Boster Bio was used for the first time. After washing the cells twice with an ice-cold flow buffer, the antibody bound to the surface protein. After binding to surface antigen, cells were analyzed with flow cytometry. Data were collected using the FlowJo program.

BeNeLux deliveries

The PAX6 protein marker is a key protein in many types of diseases, including cancer. It can also be used as a biomarker in inflammatory process. It can also be used to diagnose a number of diseases such as cancer, cardiovascular disease, neurodegenerative disorders, and cancer. This biomarker can help researchers identify the cells responsible for the enzyme's production. Boster Bio has developed an ELISA kit that detects PAX6 using a variety of samples. This ELISA has high sensitivity, and is highly sensitive. Boster Bio is a well-known manufacturer of ELISA and antibodies. Their products are validated for use in various applications including immunology, developmental biology, neurosciences, inflammation, and cancer. Boster Bio's antibodies are also validated quantitatively compared to a panel.

Boster Bio provides anti-PAX6 antibodies that react with Human, Mouse, and Rat. The antibody can keep at 4°C for 1 month. The antibody contains 5mg BSA as well as the middle region of human PAX6 protein. Blocking peptides that are based on the immunogen's length can also be purchased. The PAX6 monoclonal Antibody comes with a BSA Free version.