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Product Info Summary
| SKU: | A00273-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-PAX6 Antibody (Center)
SKU/Catalog Number
A00273-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-PAX6 Antibody (Center) (Catalog # A00273-1). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PAX6 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00273-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00273-1 is reactive to PAX6 in Human
Reconstitution
Calculated molecular weight
46683 Da
Background of PAX6
PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00273-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of PAX6 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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PAX6 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For PAX6 (Source: Uniprot.org, NCBI)
Gene Name
PAX6
Full Name
Paired box protein Pax-6
Weight
46683 Da
Superfamily
paired homeobox family
Alternative Names
Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2 PAX6 AN, AN1, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR paired box 6 paired box protein Pax-6|Aniridia 1|Aniridia 2|aniridia type II protein|oculorhombin|paired box homeotic gene-6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX6, check out the PAX6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX6 Antibody (Center) (A00273-1)
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