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Product Info Summary
| SKU: | M00273-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PAX6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00273-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAX6 Rabbit Monoclonal Antibody catalog # M00273-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00273-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HGF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAX6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00273-3 is reactive to PAX6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
46683 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00273-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of PAX6 expression in Human brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For PAX6 (Source: Uniprot.org, NCBI)
Gene Name
PAX6
Full Name
Paired box protein Pax-6
Weight
46683 MW
Superfamily
paired homeobox family
Alternative Names
Paired box protein Pax-6;Aniridia type II protein;Oculorhombin;PAX6;AN2; PAX6 AN, AN1, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR paired box 6 paired box protein Pax-6|Aniridia 1|Aniridia 2|aniridia type II protein|oculorhombin|paired box homeotic gene-6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX6, check out the PAX6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX6 Rabbit Monoclonal Antibody (M00273-3)
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No publications found for M00273-3
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PAX6 Rabbit Monoclonal Antibody
Question
I would like to test anti-PAX6 Rabbit Monoclonal antibody M00273-3 on human cerebellum for research purposes, then I may be interested in using anti-PAX6 Rabbit Monoclonal antibody M00273-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The products we sell, including anti-PAX6 Rabbit Monoclonal antibody M00273-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-01
Question
Would M00273-3 anti-PAX6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
You can see on the product datasheet, M00273-3 anti-PAX6 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use your anti-PAX6 Rabbit Monoclonal antibody for WB for human cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cerebellum identification?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
You can see on the product datasheet, M00273-3 anti-PAX6 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
My question regarding product M00273-3, anti-PAX6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00273-3 anti-PAX6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-13
Question
Is there a BSA free version of anti-PAX6 Rabbit Monoclonal antibody M00273-3 available?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAX6 Rabbit Monoclonal antibody M00273-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-08
Question
I see that the anti-PAX6 Rabbit Monoclonal antibody M00273-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-PAX6 Rabbit Monoclonal antibody M00273-3. Let me know if you need anything else.
D. Jackson
Verified customer
Asked: 2019-07-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-30
Question
Here is the WB image, lot number and protocol we used for cerebellum using anti-PAX6 Rabbit Monoclonal antibody M00273-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-15
Question
We are currently using anti-PAX6 Rabbit Monoclonal antibody M00273-3 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The anti-PAX6 Rabbit Monoclonal antibody (M00273-3) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-10
Question
We purchased anti-PAX6 Rabbit Monoclonal antibody for WB on fetal eye a few months ago. I am using rat, and We want to use the antibody for IF next. I am interested in examining fetal eye as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I looked at the website and datasheets of our anti-PAX6 Rabbit Monoclonal antibody and it appears that M00273-3 has been tested on rat in both WB and IF. Thus M00273-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-09
Question
Would anti-PAX6 Rabbit Monoclonal antibody M00273-3 work on pig IF with lung?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
Our lab technicians have not tested anti-PAX6 Rabbit Monoclonal antibody M00273-3 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PAX6 Rabbit Monoclonal antibody M00273-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lung in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-16
Question
Is a blocking peptide available for product anti-PAX6 Rabbit Monoclonal antibody (M00273-3)?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
We do provide the blocking peptide for product anti-PAX6 Rabbit Monoclonal antibody (M00273-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-17
Question
Is this M00273-3 anti-PAX6 Rabbit Monoclonal antibody reactive to the isotypes of PAX6?
M. Huang
Verified customer
Asked: 2017-05-03
Answer
The immunogen of M00273-3 anti-PAX6 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAX6 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-03
Question
We were content with the WB result of your anti-PAX6 Rabbit Monoclonal antibody. However we have observed positive staining in fetal eye nucleus. using this antibody. Is that expected? Could you tell me where is PAX6 supposed to be expressed?
A. Gonzalez
Verified customer
Asked: 2014-10-30
Answer
According to literature, fetal eye does express PAX6. Generally PAX6 expresses in nucleus. Regarding which tissues have PAX6 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Fetal eye, Pubmed ID: 1684738
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-10-30
Question
Would anti-PAX6 Rabbit Monoclonal antibody M00273-3 work for WB with cerebellum?
M. Singh
Verified customer
Asked: 2013-03-22
Answer
According to the expression profile of cerebellum, PAX6 is highly expressed in cerebellum. So, it is likely that anti-PAX6 Rabbit Monoclonal antibody M00273-3 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2013-03-22
Question
We have been able to see staining in human cerebellum. Do you have any suggestions? Is anti-PAX6 Rabbit Monoclonal antibody supposed to stain cerebellum positively?
B. Carter
Verified customer
Asked: 2013-01-31
Answer
From what I have seen in literature cerebellum does express PAX6. From what I have seen in Uniprot.org, PAX6 is expressed in palpebral conjunctiva, fetal eye, cerebellum, lung, cervix carcinoma, among other tissues. Regarding which tissues have PAX6 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Fetal eye, Pubmed ID: 1684738
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-01-31
