Anti-OIF/Ogn Antibody Picoband™

Product Name

Anti-OIF/Ogn Antibody Picoband™

View all Mimecan/OIF Antibodies

SKU/Catalog Number

A07061-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OIF/Ogn Antibody Picoband™ catalog # A07061-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-OIF/Ogn Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07061-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse OIF recombinant protein (Position: A20-F298).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07061-1 is reactive to OGN in Mouse, Rat

Applications

A07061-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

Background of Mimecan/OIF

Osteoglycin (also called mimecan), encoded by the OGN gene, is a human protein. This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml
ELISA (Cap),1-5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OIF using anti-OIF antibody (A07061-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse OGN protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIF antigen affinity purified polyclonal antibody (Catalog # A07061-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIF at approximately 34KD. The expected band size for OIF is at 34KD.

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Figure 2. Sandwich ELISA - Recombinant mouse OIF/Ogn protein standard curve.
Use in combination with reagents from Mouse OIF/Ogn ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1617).

Gene/Protein Information For OGN (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp586P2421; KSPG25; mimecan proteoglycan; Mimecan; OGN; OIF; OIFOG; osteoglycin (osteoinductive factor); osteoglycin; Osteoinductive factor; SLRR3A; SLRR3Acorneal keratan sulfate proteoglycan OGN OG, OIF, SLRR3A osteoglycin mimecan|corneal keratan sulfate proteoglycan|mimecan proteoglycan|osteoinductive factor

For more info on OGN, check out the OGN Infographic

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In this infographic, you will see the following information for OGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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