Anti-PAK1 Antibody Picoband®

Product Name

Anti-PAK1 Antibody Picoband®

View all Pak1 Antibodies

SKU/Catalog Number

PA1563

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAK1 Antibody catalog # PA1563. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PAK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1563)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PAK1, different from the related mouse and rat sequences by on amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1563 is reactive to PAK1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

40 kDa

Calculated molecular weight

60647 MW

Background of Pak1

Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activated kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined. The PAK1 gene is mapped to 11q13-q14 by inclusion within a mapped clone.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1563 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Testis Tissue, Rat Spleen Tissue, SW620 Cell, MCF-7 Cell, CEM Cell, HT1080 Cell

Validation Images & Assay Conditions

Gene/Protein Information For PAK1 (Source: Uniprot.org, NCBI)

Alternative Names

Serine/threonine-protein kinase PAK 1;2.7.11.1;Alpha-PAK;p21-activated kinase 1;PAK-1;p65-PAK;PAK1; PAK1 IDDMSSD, PAKalpha, alpha-PAK, p65-PAK p21 (RAC1) activated kinase 1 serine/threonine-protein kinase PAK 1|STE20 homolog, yeast|p21 protein (Cdc42/Rac)-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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