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Product Info Summary
| SKU: | EKC2102 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
PAK1 (Phospho-Thr212) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2102
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The PAK1 (Phospho-Thr212) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PAK1 (Phospho-Thr212) protein expression profile in cells. The kit can be used for measuring the relative amounts of PAK1 (Phospho-Thr212) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PAK1 (Phospho-Thr212).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PAK1 (Phospho-Thr212) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2102)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2102 is reactive to PAK1 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2102 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-PAK1 Phospho-Thr212 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-PAK1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells treated with etoposide 25uM 1h
Protein Target Info & Infographic
Gene/Protein Information For PAK1 (Source: Uniprot.Org, NCBI)
Gene Name
PAK1
Full Name
Serine/threonine-protein kinase PAK 1
Weight
60647 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase PAK 1;p21-activated kinase 1;PAK-1;p65-PAK;Alpha-PAK;PAK1 PAK1 IDDMSSD, PAKalpha, alpha-PAK, p65-PAK p21 (RAC1) activated kinase 1 serine/threonine-protein kinase PAK 1|STE20 homolog, yeast|p21 protein (Cdc42/Rac)-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PAK1 , check out the PAK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PAK1 : database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PAK1 (Phospho-Thr212) Colorimetric Cell-Based ELISA Kit (EKC2102)
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