Anti-CtBP1 Antibody Picoband®

CTBP1 antibody

Boster Bio Anti-CtBP1 Antibody Picoband® catalog # PB9425. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9425
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-CtBP1 Antibody Picoband®

View all CTBP1 Antibodies

SKU/Catalog Number

PB9425

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CtBP1 Antibody Picoband® catalog # PB9425. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CtBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9425)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9425 is reactive to CTBP1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

48 kDa

Calculated molecular weight

47535 MW

Background of CTBP1

CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16.This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9425 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human SH-SY5Y whole cell, human MOLT-4 whole cell, human MCF-7 whole cell, rat PC-12 whole cell, mouse SP2/0 whole cell
IHC: Mouse Thymus tissue, Rat Spleen tissue, Human Tonsil tissue
ICC/IF: U20S cell, T47D cell
IF: human intestinal cancer tissue, rat intestine tissue
FCM: U20S cell

Validation Images & Assay Conditions

Gene/Protein Information For CTBP1 (Source: Uniprot.org, NCBI)

Gene Name

CTBP1

Full Name

C-terminal-binding protein 1

Weight

47535 MW

Superfamily

D-isomer specific 2-hydroxyacid dehydrogenase family

Alternative Names

C-terminal-binding protein 1;CtBP1;1.1.1.-;CTBP1;CTBP; CTBP1 BARS, HADDTS C-terminal binding protein 1 C-terminal-binding protein 1|brefeldin A-ribosylated substrate

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CTBP1, check out the CTBP1 Infographic

CTBP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CTBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-CtBP1 Antibody Picoband®

Question

Is this PB9425 anti-CtBP1 antibody reactive to the isotypes of CTBP1?

D. Miller

Verified customer

Asked: 2020-03-20

Answer

The immunogen of PB9425 anti-CtBP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1 (409-440aa HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-20

Question

We are currently using anti-CtBP1 antibody PB9425 for mouse tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

B. Thomas

Verified customer

Asked: 2016-08-30

Answer

The anti-CtBP1 antibody (PB9425) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-30

Order DetailsPrice
PB9425

100μg

$370
PB9425-10ug

10μg sample (liquid)

$99
PB9425-Biotin

100 μg Biotin conjugated

$570
PB9425-Cy3

100 μg Cy3 conjugated

$570
PB9425-Dylight488

100 μg Dylight488 conjugated

$570
PB9425-Dylight550

100 μg Dylight550 conjugated

$570
PB9425-Dylight594

100 μg Dylight594 conjugated

$570
PB9425-FITC

100 μg FITC conjugated

$570
PB9425-HRP

100 μg HRP conjugated

$570
PB9425-APC

100 μg APC conjugated

$670
PB9425-PE

100 μg PE conjugated

$670
PB9425-iFluor647

100 μg iFluor647 conjugated

$670
PB9425-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9425
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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