Product Info Summary
SKU: | PB9425 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CtBP1 Antibody Picoband®
SKU/Catalog Number
PB9425
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CtBP1 Antibody Picoband® catalog # PB9425. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CtBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9425)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9425 is reactive to CTBP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
47535 MW
Background of CTBP1
CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene. The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins. This gene is mapped to 4p16.This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9425 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 2μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human SH-SY5Y whole cell, human MOLT-4 whole cell, human MCF-7 whole cell, rat PC-12 whole cell, mouse SP2/0 whole cell
IHC: Mouse Thymus tissue, Rat Spleen tissue, Human Tonsil tissue
ICC/IF: U20S cell, T47D cell
IF: human intestinal cancer tissue, rat intestine tissue
FCM: U20S cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CTBP1 using anti-CTBP1 antibody (PB9425).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP1 antigen affinity purified polyclonal antibody (Catalog # PB9425) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTBP1 at approximately 48 kDa. The expected band size for CTBP1 is at 48 kDa.
Click image to see more details
Figure 2. IHC analysis of CTBP1 using anti-CTBP1 antibody (PB9425).
CTBP1 was detected in paraffin-embedded section of Mouse Thymus Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CTBP1 using anti-CTBP1 antibody (PB9425).
CTBP1 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CTBP1 using anti-CTBP1 antibody (PB9425).
CTBP1 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425)
CTBP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425)
CTBP1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425).
CTBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 8. IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425) and anti-Tubulin Alpha antibody (M03989-3).
CTBP1 was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-CTBP1 Antibody (PB9425) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 9. Flow Cytometry analysis of U20S cells using anti-CTBP1 antibody (PB9425).
Overlay histogram showing U20S cells stained with PB9425 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP1 Antibody (PB9425,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CTBP1 (Source: Uniprot.org, NCBI)
Gene Name
CTBP1
Full Name
C-terminal-binding protein 1
Weight
47535 MW
Superfamily
D-isomer specific 2-hydroxyacid dehydrogenase family
Alternative Names
C-terminal-binding protein 1;CtBP1;1.1.1.-;CTBP1;CTBP; CTBP1 BARS, HADDTS C-terminal binding protein 1 C-terminal-binding protein 1|brefeldin A-ribosylated substrate
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTBP1, check out the CTBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CtBP1 Antibody Picoband® (PB9425)
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Customer Q&As
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2 Customer Q&As for Anti-CtBP1 Antibody Picoband®
Question
Is this PB9425 anti-CtBP1 antibody reactive to the isotypes of CTBP1?
D. Miller
Verified customer
Asked: 2020-03-20
Answer
The immunogen of PB9425 anti-CtBP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1 (409-440aa HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-20
Question
We are currently using anti-CtBP1 antibody PB9425 for mouse tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
B. Thomas
Verified customer
Asked: 2016-08-30
Answer
The anti-CtBP1 antibody (PB9425) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-30