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Product Info Summary
| SKU: | M00583-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-(Mouse) Suz12 Antibody (Center)
SKU/Catalog Number
M00583-2
Size
80 µl
Description
Boster Bio Anti-(Mouse) Suz12 Antibody (Center) (Catalog # M00583-2). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Suz12 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00583-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This mouse Suz12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 381-395 amino acids from the Central region of mouse Suz12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00583-2 is reactive to SUZ12 in Human, Mouse
Applications
M00583-2 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
83026 Da
Background of SUZ12
Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-Suz12 Antibody (Center) at 1:2000 dilution
Lane 1: mouse spleen lysates
Lane 2: mouse cerebellum lysates
Lane 3: Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M00583-2 staining (Mouse) Suz12 in mouse spleen sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For SUZ12 (Source: Uniprot.org, NCBI)
Gene Name
SUZ12
Full Name
Polycomb protein SUZ12
Weight
83026 Da
Superfamily
VEFS (VRN2-EMF2-FIS2-SU(Z)12) family
Alternative Names
ChET 9 protein; CHET9; CHET9polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein; JJAZ1; JJAZ1Joined to JAZF1 protein; KIAA0160Suppressor of zeste 12 protein homolog; suppressor of zeste 12 homolog (Drosophila); SUZ12 SUZ12 CHET9, IMMAS, JJAZ1 SUZ12 polycomb repressive complex 2 subunit polycomb protein SUZ12|chET 9 protein|chromatin precipitated E2F target 9 protein|joined to JAZF1 protein|suppressor of zeste 12 protein homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUZ12, check out the SUZ12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUZ12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-(Mouse) Suz12 Antibody (Center) (M00583-2)
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