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Product Info Summary
| SKU: | PA1822 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LAMP1 Antibody Picoband®
View all LAMP-1/CD107a Antibodies
SKU/Catalog Number
PA1822
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LAMP1 Antibody catalog # PA1822. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LAMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1822)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1822 is reactive to LAMP1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-120 kDa (Glycosylation)
Calculated molecular weight
44882 MW
Background of LAMP-1/CD107a
LAMP1 (lysosomal-associated membrane protein 1) also called LAMPA, LGP120 or CD107A, is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells. By means of in situ hybridization, Mattei et al. (1990) assigned the LAMP1 gene to chromosome 13q34. A related gene, which may be a pseudogene, mapped to chromosome 12p13.3. Hybridization of LAMP1 cDNA to chromosome 12p13.3 was observed even when probes representing different portions of the LAMP1 cDNA were used.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1822 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: human HepG2 whole cell, human Jurkat whole cell, human Hela whole cell, mouse brain tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LAMP1 using anti-LAMP1 antibody (PA1822).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP1 antigen affinity purified polyclonal antibody (Catalog # PA1822) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP1 at approximately 90-120 kDa (Glycosylation). The expected band size for LAMP1 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LAMP1 (Source: Uniprot.org, NCBI)
Gene Name
LAMP1
Full Name
Lysosome-associated membrane glycoprotein 1
Weight
44882 MW
Superfamily
LAMP family
Alternative Names
Lysosome-associated membrane glycoprotein 1;LAMP-1;Lysosome-associated membrane protein 1;CD107 antigen-like family member A;CD107a;LAMP1; LAMP1 CD107a, LAMPA, LGP120 lysosomal associated membrane protein 1 lysosome-associated membrane glycoprotein 1|CD107 -like family member A|LAMP-1|lysosome-associated membrane protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMP1, check out the LAMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAMP1 Antibody Picoband® (PA1822)
Hello CJ!
PA1822 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma WQ,Sun XJ,Zhu Y,Liu NF. PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. Cell Death Dis.2020 Nov 17;11(11):991.doi:10.1038/s41419-020-03162-w. PMID:33203874;PMCID:PMC7673024.
Species: Rat
PA1822 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-LAMP1 Antibody Picoband®
Question
Can you help my question with product PA1822, anti-LAMP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1822 anti-LAMP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-18
Question
Do I have to kill the cells before running my WB assay with this anti-LAMP1 antibody?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
No. The WB assay with this antibody is designed to detect live cells.
Boster Scientific Support
Answered: 2020-02-27
Question
Is this PA1822 anti-LAMP1 antibody reactive to the isotypes of LAMP1?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The immunogen of PA1822 anti-LAMP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1(403-417aa YLVGRKRSHAGYQTI), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-08
Question
We have been able to see staining in rat plasma. Do you have any suggestions? Is anti-LAMP1 antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Based on literature plasma does express LAMP1. Based on Uniprot.org, LAMP1 is expressed in nephron tubule, mammary gland, brain, pancreas testis, placenta, plasma, liver, among other tissues. Regarding which tissues have LAMP1 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 14702039
Placenta, Pubmed ID: 17897319, 8517882, 2584229, 2243102, 8323299
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-11-06
Question
I was wanting to use using your anti-LAMP1 antibody for regulation of organelle transport along microtubule studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
I appreciate your inquiry. This PA1822 anti-LAMP1 antibody is validated on rat testis tissue, tissue lysate, a549 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-09
Question
I am looking for to test anti-LAMP1 antibody PA1822 on human liver for research purposes, then I may be interested in using anti-LAMP1 antibody PA1822 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The products we sell, including anti-LAMP1 antibody PA1822, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-27
Question
I see that the anti-LAMP1 antibody PA1822 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-14
Question
I was wanting to use your anti-LAMP1 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
You can see on the product datasheet, PA1822 anti-LAMP1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-13
Question
We were well pleased with the WB result of your anti-LAMP1 antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is LAMP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
According to literature, placenta does express LAMP1. Generally LAMP1 expresses in cell membrane. Regarding which tissues have LAMP1 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 14702039
Placenta, Pubmed ID: 17897319, 8517882, 2584229, 2243102, 8323299
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-06-19
Question
Is there a BSA free version of anti-LAMP1 antibody PA1822 available?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LAMP1 antibody PA1822 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-24
Question
Please see the WB image, lot number and protocol we used for liver using anti-LAMP1 antibody PA1822. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-06
Question
We are currently using anti-LAMP1 antibody PA1822 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
The anti-LAMP1 antibody (PA1822) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-02
Question
Is a blocking peptide available for product anti-LAMP1 antibody (PA1822)?
J. Krishna
Verified customer
Asked: 2017-01-30
Answer
We do provide the blocking peptide for product anti-LAMP1 antibody (PA1822). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-01-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-LAMP1 antibody PA1822. Let me know if you need anything else.
D. Carter
Verified customer
Asked: 2016-05-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-25
Question
Will anti-LAMP1 antibody PA1822 work for WB with liver?
K. Krishna
Verified customer
Asked: 2015-06-03
Answer
According to the expression profile of liver, LAMP1 is highly expressed in liver. So, it is likely that anti-LAMP1 antibody PA1822 will work for WB with liver.
Boster Scientific Support
Answered: 2015-06-03
Question
Would PA1822 anti-LAMP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Krishna
Verified customer
Asked: 2015-01-28
Answer
It shows on the product datasheet, PA1822 anti-LAMP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-01-28
Question
Does anti-LAMP1 antibody PA1822 work on dog WB with brain?
Z. Carter
Verified customer
Asked: 2013-06-21
Answer
Our lab technicians have not tested anti-LAMP1 antibody PA1822 on dog. You can run a BLAST between dog and the immunogen sequence of anti-LAMP1 antibody PA1822 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-21
