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Key=name, value=Anti-NMI Antibody Picoband® (monoclonal, 2F3)
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Key=meta_title, value=Anti-NMI Antibody Picoband® (monoclonal, 2F3) | Bosterbio
Key=meta_description, value=Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of NMI using anti-NMI antibody (M02768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample und
Key=small_image_label, value=Figure 1. Western blot analysis of NMI using anti-NMI antibody (M02768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample und
Key=thumbnail_label, value=Figure 1. Western blot analysis of NMI using anti-NMI antibody (M02768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample und
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Key=size, value=100 μg/vial
Key=gene_full_name, value=N-myc (and STAT) interactor
Key=uniprot_id, value=Q13287
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Key=sensitivity
Key=applications, value=Flow Cytometry, IF, IHC, ICC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
Key=clone_number, value=2F3
Key=predicted_reactivity
Key=source_company, value=Boster M02768
Key=isotype, value=Mouse IgG2b
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies, Monoclonal Antibodies
Key=research_category, value=Cytoskeleton, Cytoskeleton/ECM, Motor Proteins, Myosin, Signal Transduction
Key=clonality, value=Monoclonal
Key=swatch_image, value=/m/0/m02755-2-mcm6-primary-antibodies-wb-testing-1.jpg
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Key=description, value=Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-NMI Antibody Picoband® (monoclonal, 2F3)
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
Key=gene_name, value=NMI
Key=synonyms, value=N-myc-interactor; Nmi; N-myc and STAT interactor; NMI
Key=application_details, value=Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells

Key=subcellular_localization, value=Cytoplasm.
Key=tissue_specificity, value=Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.
Key=protein_function, value=May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=40 kDa
Key=custom_attribute_2, value=WB: human placenta tissue, K562 whole cell, Hela whole cell, A431 whole cell, PC-3 whole cell, Caco-2 whole cell, HEK293 whole cell, mouse intestine tissue, rat liver tissue
IHC: human lung cancer tissue, human colon cancer tissue, human placenta tissue
ICC/IF: SiHa cell
FCM: A431 cell
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Anti-NMI Antibody Picoband® (monoclonal, 2F3)

N myc interactor antibody

Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M02768
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-NMI Antibody Picoband® (monoclonal, 2F3)

View all N myc interactor Antibodies

SKU/Catalog Number

M02768

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NMI Antibody Picoband® (monoclonal, 2F3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02768)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

2F3

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02768 is reactive to NMI in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

40 kDa

Calculated molecular weight

35.057kDa

Background of N myc interactor

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M02768 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells

Positive Control

WB: human placenta tissue, K562 whole cell, Hela whole cell, A431 whole cell, PC-3 whole cell, Caco-2 whole cell, HEK293 whole cell, mouse intestine tissue, rat liver tissue
IHC: human lung cancer tissue, human colon cancer tissue, human placenta tissue
ICC/IF: SiHa cell
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For NMI (Source: Uniprot.org, NCBI)

Gene Name

NMI

Full Name

N-myc-interactor

Weight

35.057kDa

Superfamily

NMI family

Alternative Names

N-myc-interactor; Nmi; N-myc and STAT interactor; NMI NMI N-myc and STAT interactor N-myc-interactor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NMI, check out the NMI Infographic

NMI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NMI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-NMI Antibody Picoband® (monoclonal, 2F3)

Question

We want to test anti-NMI antibody (monoclonal, 2F3) M02768 on rat bone marrow for research purposes, then I may be interested in using anti-NMI antibody (monoclonal, 2F3) M02768 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

The products we sell, including anti-NMI antibody (monoclonal, 2F3) M02768, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-13

Question

Is a blocking peptide available for product anti-NMI antibody (monoclonal, 2F3) (M02768)?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

We do provide the blocking peptide for product anti-NMI antibody (monoclonal, 2F3) (M02768). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-13

Question

Is there a BSA free version of anti-NMI antibody (monoclonal, 2F3) M02768 available?

Verified Customer

Verified customer

Asked: 2018-10-31

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NMI antibody (monoclonal, 2F3) M02768 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-31

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-NMI antibody (monoclonal, 2F3) M02768. Let me know if you need anything else.

B. Kulkarni

Verified customer

Asked: 2016-12-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-12-26

Question

I was wanting to use your anti-NMI antibody (monoclonal, 2F3) for IHC-P for rat bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat bone marrow identification?

A. Mangal

Verified customer

Asked: 2015-06-11

Answer

It shows on the product datasheet, M02768 anti-NMI antibody (monoclonal, 2F3) has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-06-11

Order DetailsPrice
M02768

100μg

$370
M02768-10ug

10μg sample (liquid)

$99
M02768-Biotin

100 μg Biotin conjugated

$570
M02768-Cy3

100 μg Cy3 conjugated

$570
M02768-Dylight488

100 μg Dylight488 conjugated

$570
M02768-Dylight550

100 μg Dylight550 conjugated

$570
M02768-Dylight594

100 μg Dylight594 conjugated

$570
M02768-FITC

100 μg FITC conjugated

$570
M02768-HRP

100 μg HRP conjugated

$570
M02768-APC

100 μg APC conjugated

$670
M02768-PE

100 μg PE conjugated

$670
M02768-iFluor647

100 μg iFluor647 conjugated

$670
M02768-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M02768
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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