Product Info Summary
SKU: | M02768 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NMI Antibody Picoband® (monoclonal, 2F3)
View all N myc interactor Antibodies
SKU/Catalog Number
M02768
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NMI Antibody Picoband® (monoclonal, 2F3) catalog # M02768. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NMI Antibody Picoband® (monoclonal, 2F3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02768)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2F3
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02768 is reactive to NMI in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
35.057kDa
Background of N myc interactor
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02768 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human placenta tissue, K562 whole cell, Hela whole cell, A431 whole cell, PC-3 whole cell, Caco-2 whole cell, HEK293 whole cell, mouse intestine tissue, rat liver tissue
IHC: human lung cancer tissue, human colon cancer tissue, human placenta tissue
ICC/IF: SiHa cell
FCM: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NMI using anti-NMI antibody (M02768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: K562 whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: A431 whole cell lysates,
Lane 5: PC-3 whole cell lysates,
Lane 6: Caco-2 whole cell lysates,
Lane 7: HEK293 whole cell lysates,
Lane 8: mouse intestine tissue lysates,
Lane 9: rat liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NMI antigen affinity purified monoclonal antibody (Catalog # M02768) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NMI at approximately 40KD. The expected band size for NMI is at 35KD.
Click image to see more details
Figure 2. IHC analysis of NMI using anti-NMI antibody (M02768).
NMI was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NMI Antibody (M02768) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of NMI using anti-NMI antibody (M02768).
NMI was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NMI Antibody (M02768) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of NMI using anti-NMI antibody (M02768).
NMI was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NMI Antibody (M02768) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of NMI using anti-NMI antibody (M02768).
NMI was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-NMI Antibody (M02768) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of A431 cells using anti- NMI antibody (M02768).
Overlay histogram showing A431 cells stained with M02768 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NMI Antibody (M02768, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NMI (Source: Uniprot.org, NCBI)
Gene Name
NMI
Full Name
N-myc-interactor
Weight
35.057kDa
Superfamily
NMI family
Alternative Names
N-myc-interactor; Nmi; N-myc and STAT interactor; NMI NMI N-myc and STAT interactor N-myc-interactor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NMI, check out the NMI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NMI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NMI Antibody Picoband® (monoclonal, 2F3) (M02768)
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Customer Q&As
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5 Customer Q&As for Anti-NMI Antibody Picoband® (monoclonal, 2F3)
Question
We want to test anti-NMI antibody (monoclonal, 2F3) M02768 on rat bone marrow for research purposes, then I may be interested in using anti-NMI antibody (monoclonal, 2F3) M02768 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The products we sell, including anti-NMI antibody (monoclonal, 2F3) M02768, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-13
Question
Is a blocking peptide available for product anti-NMI antibody (monoclonal, 2F3) (M02768)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We do provide the blocking peptide for product anti-NMI antibody (monoclonal, 2F3) (M02768). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-13
Question
Is there a BSA free version of anti-NMI antibody (monoclonal, 2F3) M02768 available?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NMI antibody (monoclonal, 2F3) M02768 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-NMI antibody (monoclonal, 2F3) M02768. Let me know if you need anything else.
B. Kulkarni
Verified customer
Asked: 2016-12-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-26
Question
I was wanting to use your anti-NMI antibody (monoclonal, 2F3) for IHC-P for rat bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat bone marrow identification?
A. Mangal
Verified customer
Asked: 2015-06-11
Answer
It shows on the product datasheet, M02768 anti-NMI antibody (monoclonal, 2F3) has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-11