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Product Info Summary
| SKU: | RP1110 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SR1/SRI Antibody Picoband®
SKU/Catalog Number
RP1110
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SR1/SRI Antibody catalog # RP1110. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR1/SRI Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1110)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SR1, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1110 is reactive to SRI in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
21676 MW
Background of SR1
Sorcin is a protein that in humans is encoded by the SRI gene. It is mapped to 7q21.1. This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1110 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat liver extract (lane 1), rat brain extract (lane 2) and SMMC7721 whole cell (lane 3)
Validation Images & Assay Conditions
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Western blot analysis of SRI expression in rat liver extract (lane 1), rat brain extract (lane 2) and SMMC7721 whole cell lysates (lane 3). SRI at 26KD was detected using rabbit anti-SRI Antigen Affinity purified polyclonal antibody (Catalog # RP1110) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For SRI (Source: Uniprot.org, NCBI)
Gene Name
SRI
Full Name
Sorcin
Weight
21676 MW
Alternative Names
Sorcin;22 kDa protein;CP-22;CP22;V19;SRI; SRI CP-22, CP22, SCN, V19 sorcin sorcin|22 kDa protein|H_RG167B05.1|calcium binding protein amplified in mutlidrug-resistant cells
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRI, check out the SRI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SR1/SRI Antibody Picoband® (RP1110)
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No publications found for RP1110
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Customer Q&As
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1 Customer Q&As for Anti-SR1/SRI Antibody Picoband®
Question
We are currently using anti-SR1/SRI antibody RP1110 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-SR1/SRI antibody (RP1110) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
