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Product Info Summary
| SKU: | PA2142 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor E2F3 E2F3 Antibody Picoband®
SKU/Catalog Number
PA2142
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor E2F3 E2F3 Antibody catalog # PA2142. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor E2F3 E2F3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2142)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2142 is reactive to E2F3 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
49162 MW
Background of E2F3
Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2142 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: HELA Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-E2F3 antibody, PA2142, Western blotting
All lanes: Anti E2F3 (PA2142) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
Protein Target Info & Infographic
Gene/Protein Information For E2F3 (Source: Uniprot.org, NCBI)
Gene Name
E2F3
Full Name
Transcription factor E2F3
Weight
49162 MW
Superfamily
E2F/DP family
Alternative Names
Transcription factor E2F3;E2F-3;E2F3;KIAA0075; E2F3 E2F-3 E2F transcription factor 3 transcription factor E2F3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F3, check out the E2F3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor E2F3 E2F3 Antibody Picoband® (PA2142)
Hello CJ!
PA2142 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang Py, Li Yj, Zhang S, Li Zl, Yue Z, Xie N, Xie Sy. Mol Cell Biochem. 2010 Jun;339(1-2):163-71. Doi: 10.1007/S11010-009-0380-2. Epub 2010 Jan 5. Regulating A549 Cells Growth By Aso Inhibiting Mirna Expression.
Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Transcription factor E2F3 E2F3 Antibody Picoband®
Question
Is this PA2142 anti-E2F3 antibody reactive to the isotypes of E2F3?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The immunogen of PA2142 anti-E2F3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3(446-465aa DLFDAYDLEKLPLVEDFMCS), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-01
Question
We are currently using anti-E2F3 antibody PA2142 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-E2F3 antibody (PA2142) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
Is a blocking peptide available for product anti-E2F3 antibody (PA2142)?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We do provide the blocking peptide for product anti-E2F3 antibody (PA2142). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-09
Question
Please see the WB image, lot number and protocol we used for buccal mucosa cell using anti-E2F3 antibody PA2142. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-26
Question
I see that the anti-E2F3 antibody PA2142 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-28
Question
I was wanting to use your anti-E2F3 antibody for WB for human buccal mucosa cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human buccal mucosa cell identification?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
As indicated on the product datasheet, PA2142 anti-E2F3 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-E2F3 antibody PA2142. Let me know if you need anything else.
K. Jha
Verified customer
Asked: 2016-12-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-26
