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Product Info Summary
| SKU: | PA2181-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Hyaluronidase-1 HYAL1 Antibody Picoband®
View all Hyaluronidase 1/HYAL1 Antibodies
SKU/Catalog Number
PA2181-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hyaluronidase-1 HYAL1 Antibody catalog # PA2181-1. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hyaluronidase-1 HYAL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2181-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HYAL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2181-1 is reactive to HYAL1 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
48368 MW
Background of Hyaluronidase 1/HYAL1
Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is though to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2181-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Positive Control
WB: human HepG2 whole cell, human PC-3 whole cell, monkey COS-7 whole cell, rat liver tissue, mouse liver tissue, mouse kidney tissue
IHC: Human Intestinal Cancer tissue
IHC-F: Human Placenta tissue
ICC: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HYAL1 antigen affinity purified polyclonal antibody (Catalog # PA2181-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HYAL1 at approximately 60 kDa. The expected band size for HYAL1 is at 48 kDa.
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Figure 2. IHC analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1).
HYAL1 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HYAL1 Antibody (PA2181-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. ICC analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1).
HYAL1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-HYAL1 Antibody (PA2181-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1).
HYAL1 was detected in a frozen section of Human Placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HYAL1 Antibody (PA2181-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HYAL1 (Source: Uniprot.org, NCBI)
Gene Name
HYAL1
Full Name
Hyaluronidase-1
Weight
48368 MW
Superfamily
glycosyl hydrolase 56 family
Alternative Names
Hyaluronidase-1;Hyal-1;3.2.1.35;Hyaluronoglucosaminidase-1;Lung carcinoma protein 1;LuCa-1;HYAL1;LUCA1; HYAL1 HYAL-1, LUCA1, MPS9, NAT6 hyaluronidase 1 hyaluronidase-1|hyaluronoglucosaminidase 1|luCa-1|lung carcinoma protein 1|plasma hyaluronidase|tumor suppressor LUCA-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HYAL1, check out the HYAL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HYAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hyaluronidase-1 HYAL1 Antibody Picoband® (PA2181-1)
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