Click image to see more details
Product Info Summary
| SKU: | A00620-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CSF1 Antibody Picoband®
SKU/Catalog Number
A00620-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CSF1 Antibody Picoband® catalog # A00620-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CSF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00620-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CSF1 recombinant protein (Position: E33-N190).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00620-3 is reactive to CSF1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
60.179kDa
Background of M-CSF
M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00620-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human 293T whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CSF1 using anti-CSF1 antibody (A00620-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human 293T whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF1 antigen affinity purified polyclonal antibody (Catalog # A00620-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF1 at approximately 60KD. The expected band size for CSF1 is at 60KD.
Protein Target Info & Infographic
Gene/Protein Information For CSF1 (Source: Uniprot.org, NCBI)
Gene Name
CSF1
Full Name
Macrophage colony-stimulating factor 1
Weight
60.179kDa
Alternative Names
Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim; Processed macrophage colony-stimulating factor 1; CSF1 CSF1 CSF-1, MCSF colony stimulating factor 1 macrophage colony-stimulating factor 1|colony stimulating factor 1 (macrophage)|lanimostim|macrophage colony stimulating factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSF1, check out the CSF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CSF1 Antibody Picoband® (A00620-3)
Hello CJ!
A00620-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epigenetic silencing of miR-130b in ovarian cancer promotes the development of multidrug resistance by targeting colony-stimulating factor 1
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Customer Q&As
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16 Customer Q&As for Anti-CSF1 Antibody Picoband®
Question
We have been able to see staining in mouse trophoblast urine. Are there any suggestions? Is anti-CSF1 antibody supposed to stain trophoblast urine positively?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
From what I have seen in literature trophoblast urine does express CSF1. From what I have seen in Uniprot.org, CSF1 is expressed in esophagus, pancreatic carcinoma urine, trophoblast urine, pancreatic carcinoma, t lymphoblast, placenta, kidney, endometrium, erythroleukemia, among other tissues. Regarding which tissues have CSF1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 1791839
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Pancreatic carcinoma, Pubmed ID: 2460758, 3500041
Pancreatic carcinoma, and Urine, Pubmed ID: 2996129
Placenta, Pubmed ID: 14702039
T lymphoblast, Pubmed ID: 2660794, 8262907
Trophoblast, and Urine, Pubmed ID: 3493529
Boster Scientific Support
Answered: 2020-04-15
Question
Please see the WB image, lot number and protocol we used for esophagus using anti-CSF1 antibody A00620-3. Please let me know if you require anything else.
G. Anderson
Verified customer
Asked: 2020-03-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
My boss were satisfied with the WB result of your anti-CSF1 antibody. However we have seen positive staining in pancreatic carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CSF1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to literature, pancreatic carcinoma does express CSF1. Generally CSF1 expresses in cell membrane. Regarding which tissues have CSF1 expression, here are a few articles citing expression in various tissues:
Endometrium, Pubmed ID: 1791839
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Pancreatic carcinoma, Pubmed ID: 2460758, 3500041
Pancreatic carcinoma, and Urine, Pubmed ID: 2996129
Placenta, Pubmed ID: 14702039
T lymphoblast, Pubmed ID: 2660794, 8262907
Trophoblast, and Urine, Pubmed ID: 3493529
Boster Scientific Support
Answered: 2020-01-06
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-CSF1 antibody A00620-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
I was wanting to use your anti-CSF1 antibody for ELISA for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, A00620-3 anti-CSF1 antibody has been validated for ELISA, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
Do you have a BSA free version of anti-CSF1 antibody A00620-3 available?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CSF1 antibody A00620-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-10
Question
We are currently using anti-CSF1 antibody A00620-3 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-CSF1 antibody (A00620-3) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
I would like to test anti-CSF1 antibody A00620-3 on human esophagus for research purposes, then I may be interested in using anti-CSF1 antibody A00620-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The products we sell, including anti-CSF1 antibody A00620-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-07
Question
Is this A00620-3 anti-CSF1 antibody reactive to the isotypes of CSF1?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The immunogen of A00620-3 anti-CSF1 antibody is E. coli-derived human CSF1 recombinant protein (Position: E33-N190). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-16
Question
We ordered your anti-CSF1 antibody for WB on erythroleukemia a few years ago. I am using human, and We intend to use the antibody for ELISA next. My lab would like examining erythroleukemia as well as pancreatic carcinoma urine in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
L. Parker
Verified customer
Asked: 2018-06-29
Answer
I looked at the website and datasheets of our anti-CSF1 antibody and it seems that A00620-3 has been tested on human in both WB and ELISA. Thus A00620-3 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-29
Question
Would A00620-3 anti-CSF1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
It shows on the product datasheet, A00620-3 anti-CSF1 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-05
Question
We want using your anti-CSF1 antibody for microglial cell proliferation studies. Has this antibody been tested with western blotting on 293t whole cell lysate? We would like to see some validation images before ordering.
F. Mitchell
Verified customer
Asked: 2015-10-08
Answer
I appreciate your inquiry. This A00620-3 anti-CSF1 antibody is tested on human placenta tissue, 293t whole cell lysate. It is guaranteed to work for ELISA, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-10-08
Question
Is a blocking peptide available for product anti-CSF1 antibody (A00620-3)?
B. Mangal
Verified customer
Asked: 2015-07-14
Answer
We do provide the blocking peptide for product anti-CSF1 antibody (A00620-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-14
Question
Can you help my question with product A00620-3, anti-CSF1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Miller
Verified customer
Asked: 2015-07-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00620-3 anti-CSF1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-01
Question
I see that the anti-CSF1 antibody A00620-3 works with ELISA, what is the protocol used to produce the result images on the product page?
O. Rodriguez
Verified customer
Asked: 2013-09-17
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-17
Question
Will anti-CSF1 antibody A00620-3 work for ELISA with esophagus?
G. Moore
Verified customer
Asked: 2013-04-04
Answer
According to the expression profile of esophagus, CSF1 is highly expressed in esophagus. So, it is likely that anti-CSF1 antibody A00620-3 will work for ELISA with esophagus.
Boster Scientific Support
Answered: 2013-04-04
