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Product Info Summary
| SKU: | M00401 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MUM1 Monoclonal Antibody
SKU/Catalog Number
M00401
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MUM1 Monoclonal Antibody catalog # M00401. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00401)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADOF-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MUM1 Melanoma associated antigen (mutated) 1 (MUM1, EXPAND1) is a PWWP-domain containing chromatin binding protein involved in maintaining chromatin architecture of interphase chromosomes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00401 is reactive to IRF4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
56 kDa
Calculated molecular weight
55859 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00401 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:100
FC 1:120
Positive Control
WB: Daudi cell
Validation Images & Assay Conditions
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Western blot analysis of MUM1 expression in Daudi cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IRF4 (Source: Uniprot.org, NCBI)
Gene Name
IRF4
Full Name
Interferon regulatory factor 4
Weight
55859 MW
Superfamily
IRF family
Alternative Names
TNF receptor-associated factor 2;6.3.2.-;E3 ubiquitin-protein ligase TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3;TRAF2;TRAP3; IRF4 LSIRF, MUM1, NF-EM5, SHEP8 interferon regulatory factor 4 interferon regulatory factor 4|lymphocyte-specific interferon regulatory factor|multiple myeloma oncogene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF4, check out the IRF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUM1 Monoclonal Antibody (M00401)
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No publications found for M00401
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Customer Q&As
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15 Customer Q&As for Anti-MUM1 Monoclonal Antibody
Question
Can you help my question with product M00401, anti-MUM1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00401 anti-MUM1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
We ordered your anti-MUM1 Monoclonal antibody for WB on lymph node in the past. I am using human, and We are going to use the antibody for IF next. I am interested in examining lymph node as well as skin in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
I have checked the website and datasheets of our anti-MUM1 Monoclonal antibody and it appears that M00401 has been tested on human in both WB and IF. Thus M00401 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-31
Question
Does M00401 anti-MUM1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
You can see on the product datasheet, M00401 anti-MUM1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-30
Question
We need to test anti-MUM1 Monoclonal antibody M00401 on human lymph node for research purposes, then I may be interested in using anti-MUM1 Monoclonal antibody M00401 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The products we sell, including anti-MUM1 Monoclonal antibody M00401, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-15
Question
Is this M00401 anti-MUM1 Monoclonal antibody reactive to the isotypes of IRF4?
N. Collins
Verified customer
Asked: 2019-05-08
Answer
The immunogen of M00401 anti-MUM1 Monoclonal antibody is A synthesized peptide derived from human MUM1 Melanoma associated antigen (mutated) 1 (MUM1, EXPAND1) is a PWWP-domain containing chromatin binding protein involved in maintaining chromatin architecture of interphase chromosomes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-08
Question
Is a blocking peptide available for product anti-MUM1 Monoclonal antibody (M00401)?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
We do provide the blocking peptide for product anti-MUM1 Monoclonal antibody (M00401). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-10
Question
I was wanting to use your anti-MUM1 Monoclonal antibody for WB for human lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
You can see on the product datasheet, M00401 anti-MUM1 Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-11
Question
Do you have a BSA free version of anti-MUM1 Monoclonal antibody M00401 available?
E. Miller
Verified customer
Asked: 2018-05-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MUM1 Monoclonal antibody M00401 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-02
Question
Will anti-MUM1 Monoclonal antibody M00401 work for WB with lymph node?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
According to the expression profile of lymph node, IRF4 is highly expressed in lymph node. So, it is likely that anti-MUM1 Monoclonal antibody M00401 will work for WB with lymph node.
Boster Scientific Support
Answered: 2018-02-09
Question
See below the WB image, lot number and protocol we used for lymph node using anti-MUM1 Monoclonal antibody M00401. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-25
Question
We have observed staining in human skin. Are there any suggestions? Is anti-MUM1 Monoclonal antibody supposed to stain skin positively?
P. Lewis
Verified customer
Asked: 2016-06-01
Answer
From what I have seen in literature skin does express IRF4. From what I have seen in Uniprot.org, IRF4 is expressed in endocervix, spleen, skin, lymph node, among other tissues. Regarding which tissues have IRF4 expression, here are a few articles citing expression in various tissues:
Lymph node, Pubmed ID: 12651066
Skin, Pubmed ID: 15489334
Spleen, Pubmed ID: 8921401, 9326949
Boster Scientific Support
Answered: 2016-06-01
Question
I see that the anti-MUM1 Monoclonal antibody M00401 works with WB, what is the protocol used to produce the result images on the product page?
A. Singh
Verified customer
Asked: 2015-12-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-MUM1 Monoclonal antibody M00401. Let me know if you need anything else.
N. Collins
Verified customer
Asked: 2013-12-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-12
Question
My team were well pleased with the WB result of your anti-MUM1 Monoclonal antibody. However we have observed positive staining in spleen nucleus. using this antibody. Is that expected? Could you tell me where is IRF4 supposed to be expressed?
G. Huang
Verified customer
Asked: 2013-04-30
Answer
According to literature, spleen does express IRF4. Generally IRF4 expresses in nucleus. Regarding which tissues have IRF4 expression, here are a few articles citing expression in various tissues:
Lymph node, Pubmed ID: 12651066
Skin, Pubmed ID: 15489334
Spleen, Pubmed ID: 8921401, 9326949
Boster Scientific Support
Answered: 2013-04-30
Question
We are currently using anti-MUM1 Monoclonal antibody M00401 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
A. Mangal
Verified customer
Asked: 2013-02-28
Answer
The anti-MUM1 Monoclonal antibody (M00401) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-28
