Product Info Summary
SKU: | A00401 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Interferon regulatory factor 4 IRF4 Antibody
SKU/Catalog Number
A00401
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Interferon regulatory factor 4 IRF4 Antibody (Catalog # A00401). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IRF4 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Interferon regulatory factor 4 IRF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00401)
Host
Rabbit
Contents
IRF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IRF4 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human IRF4. The immunogen is located within the last 50 amino acids of IRF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE.
Reactive Species
A00401 is reactive to IRF4 in Human, Mouse, Rat
Applications
A00401 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
51772 MW
Background of IRF4
Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Another member, IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF4 expression is tightly regulated in resting primary T cells and plays an essential role in the homeostasis and function of mature lymphocytes. IRF4 is induced by Toll-like receptor (TLR) activation and acts as a negative regulator of TLR signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IRF4 antibody can be used for detection of IRF4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
![a00401 anti interferon regulatory factor 4 irf4 antibody wb testing 1 a00401 anti interferon regulatory factor 4 irf4 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00401-anti-interferon-regulatory-factor-4-irf4-antibody-wb-testing-1.jpg)
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Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 μg/mL.
![a00401 anti interferon regulatory factor 4 irf4 antibody icc testing 2 a00401 anti interferon regulatory factor 4 irf4 antibody icc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a00401-anti-interferon-regulatory-factor-4-irf4-antibody-icc-testing-2.jpg)
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Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 μg/mL.
![a00401 anti interferon regulatory factor 4 irf4 antibody if testing 3 a00401 anti interferon regulatory factor 4 irf4 antibody if testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a00401-anti-interferon-regulatory-factor-4-irf4-antibody-if-testing-3.jpg)
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Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IRF4 (Source: Uniprot.org, NCBI)
Gene Name
IRF4
Full Name
Interferon regulatory factor 4
Weight
51772 MW
Superfamily
IRF family
Alternative Names
interferon regulatory factor 4; IRF4; IRF-4; LSIRF; LSIRFMUM1; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5 IRF4 LSIRF, MUM1, NF-EM5, SHEP8 interferon regulatory factor 4 interferon regulatory factor 4|lymphocyte-specific interferon regulatory factor|multiple myeloma oncogene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF4, check out the IRF4 Infographic
![IRF4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon regulatory factor 4 IRF4 Antibody (A00401)
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