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Product Info Summary
| SKU: | A03559 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RBM5/Luca15 Antibody
SKU/Catalog Number
A03559
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RBM5/Luca15 Antibody (Catalog # A03559). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RBM5/Luca15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03559)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RBM5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03559 is reactive to RBM5 in Human, Mouse
Reconstitution
Calculated molecular weight
92154 MW
Background of RBM5
Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
Bader S., Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
Timmer T., Genomics 60:238-240(1999).
Oh J.J., Nucleic Acids Res. 27:4008-4017(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03559 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells, using RBM5 antibody A03559.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using RBM5 antibody A03559.
Protein Target Info & Infographic
Gene/Protein Information For RBM5 (Source: Uniprot.org, NCBI)
Gene Name
RBM5
Full Name
RNA-binding protein 5
Weight
92154 MW
Superfamily
RBM5/RBM10 family
Alternative Names
RNA-binding protein 5;Protein G15;Putative tumor suppressor LUCA15;RNA-binding motif protein 5;Renal carcinoma antigen NY-REN-9;RBM5;H37, LUCA15; RBM5 G15, H37, LUCA-15, LUCA15, RMB5 RNA binding motif protein 5 RNA-binding protein 5|putative tumor suppressor LUCA15|renal carcinoma NY-REN-9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBM5, check out the RBM5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBM5/Luca15 Antibody (A03559)
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No publications found for A03559
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-RBM5/Luca15 Antibody
Question
Does A03559 anti-RBM5/Luca15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
As indicated on the product datasheet, A03559 anti-RBM5/Luca15 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-RBM5/Luca15 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
As indicated on the product datasheet, A03559 anti-RBM5/Luca15 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
Is a blocking peptide available for product anti-RBM5/Luca15 antibody (A03559)?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We do provide the blocking peptide for product anti-RBM5/Luca15 antibody (A03559). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-30
Question
See attached the WB image, lot number and protocol we used for testis using anti-RBM5/Luca15 antibody A03559. Please let me know if you require anything else.
B. Johnson
Verified customer
Asked: 2019-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-20
Question
I am interested in to test anti-RBM5/Luca15 antibody A03559 on mouse testis for research purposes, then I may be interested in using anti-RBM5/Luca15 antibody A03559 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The products we sell, including anti-RBM5/Luca15 antibody A03559, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-03
Question
Would anti-RBM5/Luca15 antibody A03559 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
According to the expression profile of testis, RBM5 is highly expressed in testis. So, it is likely that anti-RBM5/Luca15 antibody A03559 will work for WB with testis.
Boster Scientific Support
Answered: 2019-06-28
Question
We are currently using anti-RBM5/Luca15 antibody A03559 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-RBM5/Luca15 antibody (A03559) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
