Anti-BATF Antibody

Product Name

Anti-BATF Antibody

View all BATF Antibodies

SKU/Catalog Number

A04089

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BATF Antibody (Catalog # A04089). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BATF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BATF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04089)

Host

Rabbit

Contents

BATF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BATF antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BATF. The immunogen is located within the first 50 amino acids of BATF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of BATF are known to exist. Despite its predicted molecular weight, BATF often migrates at a higher than expected molecular weight.

Reactive Species

A04089 is reactive to BATF in Human, Mouse, Rat

Applications

A04089 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

14120 MW

Background of BATF

BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.

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Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.

Gene/Protein Information For BATF (Source: Uniprot.org, NCBI)

Alternative Names

basic leucine zipper transcription factor, ATF-like; BATF; BATF1basic leucine zipper transcriptional factor ATF-like; B-ATFSFA2; B-cell-activating transcription factor; SFA2; SFA-2activating transcription factor B; SF-HT-activated gene 2 protein BATF B-ATF1, SFA-2, SFA2, BATF basic leucine zipper ATF-like transcription factor basic leucine zipper transcriptional factor ATF-like|B-cell-activating transcription factor|SF-HT-activated gene 2 protein|activating transcription factor B|basic leucine zipper transcription factor, ATF-like

For more info on BATF, check out the BATF Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BATF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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