Click image to see more details
Product Info Summary
| SKU: | M00286-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-MMP2 Monoclonal Antibody
SKU/Catalog Number
M00286-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP2 Monoclonal Antibody catalog # M00286-3. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00286-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADGF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00286-3 is reactive to MMP2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
73882 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00286-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: L6 cell
Validation Images & Assay Conditions
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Western blot analysis of MMP2 expression in L6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MMP2 (Source: Uniprot.org, NCBI)
Gene Name
MMP2
Full Name
72 kDa type IV collagenase
Weight
73882 MW
Superfamily
peptidase M10A family
Alternative Names
72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;TBE-1;PEX;MMP2;CLG4A; MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP2, check out the MMP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP2 Monoclonal Antibody (M00286-3)
Hello CJ!
M00286-3 has been cited in 23 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
Species: Human,Mouse
M00286-3 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:1000
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M00286-3 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Antitumor and antimetastatic activities of Rhizoma Paridis saponins
Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1%u03B1 and autophagy in human osteosarcoma
Inactivation of lysyl oxidase by %u03B2-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Association of MMP-2 expression and prognosis in osteosarcoma patients
TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21
Recommended Resources
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MMP2 Monoclonal Antibody
Question
Do you have a BSA free version of anti-MMP2 Monoclonal antibody M00286-3 available?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP2 Monoclonal antibody M00286-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-30
Question
Our lab used your anti-MMP2 Monoclonal antibody for WB on mucosa of stomach in the past. I am using human, and I plan to use the antibody for IF next. We are interested in examining mucosa of stomach as well as melanoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
I have checked the website and datasheets of our anti-MMP2 Monoclonal antibody and it seems that M00286-3 has been tested on human in both WB and IF. Thus M00286-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-11
Question
I see that the anti-MMP2 Monoclonal antibody M00286-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-MMP2 Monoclonal antibody (M00286-3)?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We do provide the blocking peptide for product anti-MMP2 Monoclonal antibody (M00286-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-20
Question
We have seen staining in mouse mucosa of stomach. Any tips? Is anti-MMP2 Monoclonal antibody supposed to stain mucosa of stomach positively?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
According to literature mucosa of stomach does express MMP2. According to Uniprot.org, MMP2 is expressed in mucosa of stomach, teratocarcinoma testis, brain, melanoma, among other tissues. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-29
Question
Can you help my question with product M00286-3, anti-MMP2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00286-3 anti-MMP2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-MMP2 Monoclonal antibody M00286-3 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
According to the expression profile of brain, MMP2 is highly expressed in brain. So, it is likely that anti-MMP2 Monoclonal antibody M00286-3 will work for WB with brain.
Boster Scientific Support
Answered: 2019-05-24
Question
Will M00286-3 anti-MMP2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
As indicated on the product datasheet, M00286-3 anti-MMP2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-29
Question
I was wanting to use your anti-MMP2 Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
You can see on the product datasheet, M00286-3 anti-MMP2 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-16
Question
Is this M00286-3 anti-MMP2 Monoclonal antibody reactive to the isotypes of MMP2?
A. Krishna
Verified customer
Asked: 2018-02-23
Answer
The immunogen of M00286-3 anti-MMP2 Monoclonal antibody is A synthesized peptide derived from human MMP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-23
Question
We were content with the WB result of your anti-MMP2 Monoclonal antibody. However we have been able to see positive staining in teratocarcinoma testis isoform 2: cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is MMP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
From literature, teratocarcinoma testis does express MMP2. Generally MMP2 expresses in isoform 1: secreted, extracellular space,, isoform 2: cytoplasm. mitochondrion. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-06-30
Question
We are currently using anti-MMP2 Monoclonal antibody M00286-3 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
A. Taylor
Verified customer
Asked: 2016-12-02
Answer
The anti-MMP2 Monoclonal antibody (M00286-3) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-02
Question
I was wanting to use to test anti-MMP2 Monoclonal antibody M00286-3 on human brain for research purposes, then I may be interested in using anti-MMP2 Monoclonal antibody M00286-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Moore
Verified customer
Asked: 2016-04-28
Answer
The products we sell, including anti-MMP2 Monoclonal antibody M00286-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-04-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MMP2 Monoclonal antibody M00286-3. Let me know if you need anything else.
C. Roberts
Verified customer
Asked: 2014-03-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-13
Question
See attached the WB image, lot number and protocol we used for brain using anti-MMP2 Monoclonal antibody M00286-3. Please let me know if you require anything else.
M. Collins
Verified customer
Asked: 2013-04-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-12
