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Product Info Summary
| SKU: | PB9283 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MMP14 Antibody Picoband®
View all MMP-14/MT1-MMP Antibodies
SKU/Catalog Number
PB9283
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMP14 Antibody Picoband® catalog # PB9283. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9283)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9283 is reactive to MMP14 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
65894 MW
Background of MMP-14/MT1-MMP
Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9283 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, HELA Whole Cell, U87 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MMP14 Picoband antibody, PB9283, Western blotting
All lanes: Anti MMP14 (PB9283) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Protein Target Info & Infographic
Gene/Protein Information For MMP14 (Source: Uniprot.org, NCBI)
Gene Name
MMP14
Full Name
Matrix metalloproteinase-14
Weight
65894 MW
Superfamily
peptidase M10A family
Alternative Names
Matrix metalloproteinase-14;MMP-14;3.4.24.80;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14; MMP14 MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS matrix metallopeptidase 14 matrix metalloproteinase-14|matrix metallopeptidase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type-1 matrix metalloproteinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP14, check out the MMP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP14 Antibody Picoband® (PB9283)
Hello CJ!
PB9283 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway
Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...
Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.
Dong Q, Yu D, Yang Cm, Jiang B, Zhang H. Int J Exp Pathol. 2010 Aug;91(4):368-73. Doi: 10.1111/J.1365-2613.2010.00724.X. Epub 2010 Jun 24. Expression Of The Reversion-Inducing Cysteine-Rich Protein With Kazal Motifs And Matrix Metalloproteinase-14...
Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...
Xiao H, Li H, Yu G, Xiao W, Hu J, Tang K, Zeng J, He W, Zeng G, Ye Z, Xu H. Oncol Rep. 2014 Apr;31(4):1832-8. Doi: 10.3892/Or.2014.3048. Epub 2014 Feb 24. Microrna-10B Promotes Migration And Invasion Through Klf4 And Hoxd10 In Human Bladder Cancer.
Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MMP14 Antibody Picoband®
Question
Do you have a BSA free version of anti-MMP14 antibody PB9283 available?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP14 antibody PB9283 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-28
Question
We are currently using anti-MMP14 antibody PB9283 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The anti-MMP14 antibody (PB9283) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-03
Question
Our team were happy with the WB result of your anti-MMP14 antibody. However we have observed positive staining in skin membrane using this antibody. Is that expected? Could you tell me where is MMP14 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
According to literature, skin does express MMP14. Generally MMP14 expresses in membrane. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-27
Question
We have seen staining in human tongue. Are there any suggestions? Is anti-MMP14 antibody supposed to stain tongue positively?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
From what I have seen in literature tongue does express MMP14. From what I have seen in Uniprot.org, MMP14 is expressed in endocervix, placenta, lung, tongue, skin, melanoma, among other tissues. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-26
Question
I was wanting to use your anti-MMP14 antibody for WB for human skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
It shows on the product datasheet, PB9283 anti-MMP14 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-03
Question
I am interested in using your anti-MMP14 antibody for branching morphogenesis of an epithelial tube studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
I appreciate your inquiry. This PB9283 anti-MMP14 antibody is validated on human placenta tissue, tissue lysate, hela whole cell lysate, u87 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-04
Question
I see that the anti-MMP14 antibody PB9283 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-12
Question
I have a question about product PB9283, anti-MMP14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9283 anti-MMP14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
Is this PB9283 anti-MMP14 antibody reactive to the isotypes of MMP14?
J. Taylor
Verified customer
Asked: 2019-02-12
Answer
The immunogen of PB9283 anti-MMP14 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14 (557-582aa LAVFFFRRHGTPRRLLYCQRSLLDKV), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-12
Question
Please see the WB image, lot number and protocol we used for skin using anti-MMP14 antibody PB9283. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-15
Question
I would like to test anti-MMP14 antibody PB9283 on human skin for research purposes, then I may be interested in using anti-MMP14 antibody PB9283 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The products we sell, including anti-MMP14 antibody PB9283, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-MMP14 antibody PB9283. Let me know if you need anything else.
K. Thomas
Verified customer
Asked: 2017-11-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-13
Question
Is a blocking peptide available for product anti-MMP14 antibody (PB9283)?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
We do provide the blocking peptide for product anti-MMP14 antibody (PB9283). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-29
Question
Would anti-MMP14 antibody PB9283 work for WB with skin?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
According to the expression profile of skin, MMP14 is highly expressed in skin. So, it is likely that anti-MMP14 antibody PB9283 will work for WB with skin.
Boster Scientific Support
Answered: 2017-06-29
Question
Does PB9283 anti-MMP14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Singh
Verified customer
Asked: 2016-11-15
Answer
It shows on the product datasheet, PB9283 anti-MMP14 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-11-15
