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Product Info Summary
| SKU: | EK1290 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse DMP-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1290
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse DMP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Dmp1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse DMP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1290)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L17-Y503
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1290 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1290 is reactive to Dmp1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1290 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DMP-1
Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1290-CAP | Anti-Mouse Dmp1 Pre-coated 96-well strip microplate | 1 |
| EK1290-ST | Mouse Dmp1 Standard | 2 vials, 10 ng/tube |
| EK1290-DA | Mouse Dmp1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.009 | 0.136 | 0.226 | 0.465 | 0.762 | 1.192 | 1.737 | 2.053 |
Data Example Images
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Mouse DMP-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect DMP-1, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse DMP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 279 | 1719 | 4479 | 263 | 1713 | 4336 |
| Standard deviation | 17.7% | 103.14 | 344.88 | 17.6% | 130.18 | 346.88 |
| CV (%) | 5.7% | 6% | 7.7% | 6.2% | 7.6% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DMP-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 279 | 274 | 317 | 271 | 285 | 18.55 | 6.5% |
| Sample 2 | 1719 | 1758 | 1493 | 1528 | 1624 | 115.49 | 7.1% |
| Sample 3 | 4479 | 4588 | 5236 | 4685 | 4747 | 291.57 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For Dmp1 (Source: Uniprot.Org, NCBI)
Gene Name
Dmp1
Full Name
Dentin matrix acidic phosphoprotein 1
Weight
53965 MW
Alternative Names
Dentin matrix acidic phosphoprotein 1;DMP-1;Dentin matrix protein 1;AG1;Dmp1;Dmp; DMP1 ARHP, ARHR, DMP-1 dentin matrix acidic phosphoprotein 1 dentin matrix acidic phosphoprotein 1|dentin matrix protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Dmp1, check out the Dmp1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Dmp1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse DMP-1 ELISA Kit PicoKine® (EK1290)
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Customer Q&As
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6 Customer Q&As for Mouse DMP-1 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for DMP-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DMP-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-03-11
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-06
Question
Q: What is the optimal O.D. value for DMP-1 ELISA kit? I performed your DMP-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain DMP-1 even though the O.D. values are not very high?
K. Davis
Verified customer
Asked: 2019-08-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DMP-1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DMP-1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-08-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-21
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring DMP-1 using the EK1290 Mouse DMP-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-08-07
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2015-08-07
Question
Q: can I use citrate plasma as samples in Mouse DMP-1 Picokine® ELISA Kit (Catalog # EK1290)?
L. Moore
Verified customer
Asked: 2015-05-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of DMP-1 causing degradation of its protein structure. DMP-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the DMP-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-05-14
