Product Info Summary
SKU: | PA1378-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MDM2 Antibody
SKU/Catalog Number
PA1378-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MDM2 Antibody catalog # PA1378-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1378-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1378-1 is reactive to MDM2 in Human
Applications
PA1378-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
55233 MW
Background of MDM2/HDM2
Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al. (1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (PA1378-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # PA1378-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 55KD.
Protein Target Info & Infographic
Gene/Protein Information For MDM2 (Source: Uniprot.org, NCBI)
Gene Name
MDM2
Full Name
E3 ubiquitin-protein ligase Mdm2
Weight
55233 MW
Superfamily
MDM2/MDM4 family
Alternative Names
ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 MDM2 ACTFS, HDMX, LSKB, hdm2 MDM2 proto-oncogene E3 ubiquitin-protein ligase Mdm2|MDM2 oncogene, E3 ubiquitin protein ligase|MDM2 proto-oncogene, E3 ubiquitin protein ligase|Mdm2, p53 E3 ubiquitin protein ligase homolog|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|oncoprotein Mdm2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDM2, check out the MDM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDM2 Antibody (PA1378-1)
Hello CJ!
PA1378-1 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
PA1378-1 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
Epigenetic repression of long non-coding RNA MEG3 mediated by DNMT1 represses the p53 pathway in gliomas
Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer
Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway
The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer
Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y. Oncol Rep. 2014 Dec;32(6):2703-9. Doi: 10.3892/Or.2014.3537. Epub 2014 Oct 10. Cd38 Is Highly Expressed And Affects The Pi3K/Akt Signaling Pathway In Cervical Cancer.
Xiao S, Zhou Y, Yi W, Luo G, Jiang B, Tian Q, Li Y, Xue M. Int J Oncol. 2015 Apr;46(4):1677-84. Doi: 10.3892/Ijo.2015.2873. Epub 2015 Feb 4. Fra-1 Is Downregulated In Cervical Cancer Tissues And Promotes Cervical Cancer Cell Apoptosis By P53 Signa...
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Customer Reviews
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1 Reviews For Anti-MDM2 Antibody
0
Boster's Anti-MDM2 Antibody Worked!--Stacy Wilson, Biology, REVOLUTION Medicines, Inc., SRA
Excellent
Source: Biocompare.com
Applications | Western Blot |
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Sample | MDA-MB-468 and HEK293E cell lysates |
Detection | SuperSignal West Pico Plus with a BioRad imager |
"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."
Stacy Wilson
Verified customer
Submitted 2017-08-21
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-MDM2 Antibody
Question
Is a blocking peptide available for product anti-MDM2 antibody (PA1378-1)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We do provide the blocking peptide for product anti-MDM2 antibody (PA1378-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-07
Question
Our team were content with the WB result of your anti-MDM2 antibody. However we have been able to see positive staining in rhabdomyosarcoma nucleus using this antibody. Is that expected? Could you tell me where is MDM2 supposed to be expressed?
M. Evans
Verified customer
Asked: 2020-01-21
Answer
According to literature, rhabdomyosarcoma does express MDM2. Generally MDM2 expresses in nucleus, nucleoplasm. cytoplasm. nucleus,. Regarding which tissues have MDM2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1614537
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Ovarian carcinoma, Pubmed ID: 8705862
Rhabdomyosarcoma, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-21
Question
Will anti-MDM2 antibody PA1378-1 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
According to the expression profile of erythroleukemia, MDM2 is highly expressed in erythroleukemia. So, it is likely that anti-MDM2 antibody PA1378-1 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2020-01-16
Question
Do you have a BSA free version of anti-MDM2 antibody PA1378-1 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MDM2 antibody PA1378-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
I was wanting to use your anti-MDM2 antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
As indicated on the product datasheet, PA1378-1 anti-MDM2 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-MDM2 antibody PA1378-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
Does anti-MDM2 antibody PA1378-1 work on monkey WB with colon?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Our lab technicians have not tested anti-MDM2 antibody PA1378-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-MDM2 antibody PA1378-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
We have seen staining in human colon. Do you have any suggestions? Is anti-MDM2 antibody supposed to stain colon positively?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Based on literature colon does express MDM2. Based on Uniprot.org, MDM2 is expressed in oviduct epithelium, colon, ovarian carcinoma, tongue, rhabdomyosarcoma, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have MDM2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1614537
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Ovarian carcinoma, Pubmed ID: 8705862
Rhabdomyosarcoma, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-05
Question
Please see the WB image, lot number and protocol we used for erythroleukemia using anti-MDM2 antibody PA1378-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-23
Question
Will PA1378-1 anti-MDM2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Jones
Verified customer
Asked: 2018-05-23
Answer
You can see on the product datasheet, PA1378-1 anti-MDM2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-23
Question
I was wanting to use to test anti-MDM2 antibody PA1378-1 on human erythroleukemia for research purposes, then I may be interested in using anti-MDM2 antibody PA1378-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
The products we sell, including anti-MDM2 antibody PA1378-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-02
Question
Is this PA1378-1 anti-MDM2 antibody reactive to the isotypes of MDM2?
H. Lewis
Verified customer
Asked: 2017-09-01
Answer
The immunogen of PA1378-1 anti-MDM2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2(343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-01
Question
I see that the anti-MDM2 antibody PA1378-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-04
Question
I have a question about product PA1378-1, anti-MDM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Dhar
Verified customer
Asked: 2017-03-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1378-1 anti-MDM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-03
Question
We are currently using anti-MDM2 antibody PA1378-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
J. Wu
Verified customer
Asked: 2013-11-15
Answer
The anti-MDM2 antibody (PA1378-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-15