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Product Info Summary
| SKU: | M00001 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | African green monkey, Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-P53 Antibody
SKU/Catalog Number
M00001
Size
50 µl
Description
Boster Bio Anti-P53 Antibody (Catalog # M00001). Tested in WB, IHC-P application(s). This antibody reacts with Human, African green monkey.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-P53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1711CT184.18.1
Isotype
IgG1,k
Immunogen
This P53 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 1-393 amino acids from human P53.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00001 is reactive to TP53 in African green monkey, Human
Reconstitution
Calculated molecular weight
43653 Da
Background of p53
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00001 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:400
WB: 1:2000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human Colon cancer section using Pink1. M00001 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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All lanes : Anti-P53 Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: HT-29 whole cell lysate
Lane 3: CCRF-CEM whole cell lysate
Lane 4: COS-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
43653 Da
Superfamily
p53 family
Alternative Names
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor p53| NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P53 Antibody (M00001)
Hello CJ!
M00001 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).
Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
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