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Product Info Summary
| SKU: | M00001-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p53 TP53 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00001-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p53 TP53 Rabbit Monoclonal Antibody catalog # M00001-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p53 TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AGC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p53
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00001-1 is reactive to TP53 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
53 kDa
Calculated molecular weight
53 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00001-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human PC-9 whole cell, human A431 whole cell, human 293T whole cell
IHC: human colon cancer tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p53 using anti-p53 antibody (M00001-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-9 (WT) whole cell lysates,
Lane 2: human PC-9 (KO) whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p53 antigen affinity purified monoclonal antibody (M00001-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p53 at approximately 53 kDa. The expected band size for p53 is at 53 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using p53 Antibody.
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Immunofluorescent analysis of Hela cells, using p53 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
53 kDa
Superfamily
p53 family
Alternative Names
Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;TP53;P53; TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor p53| NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p53 TP53 Rabbit Monoclonal Antibody (M00001-1)
Hello CJ!
M00001-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
M00001-1 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00001-1 usage in article: APP:IF, SAMPLE:A431 CELLS, DILUTION:NA
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).
Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-p53 TP53 Rabbit Monoclonal Antibody
Question
We ordered your anti-p53 Rabbit Monoclonal antibody for IF on glial cell glial tumor a few years ago. I am using human, and We are going to use the antibody for IP next. I would like examining glial cell glial tumor as well as ovarian adenocarcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
I took a look at the website and datasheets of our anti-p53 Rabbit Monoclonal antibody and I see that M00001-1 has been tested on human in both IF and IP. Thus M00001-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-p53 Rabbit Monoclonal antibody M00001-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
The anti-p53 Rabbit Monoclonal antibody (M00001-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-13
Question
We have seen staining in human lung carcinoma. Are there any suggestions? Is anti-p53 Rabbit Monoclonal antibody supposed to stain lung carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
From literature lung carcinoma does express TP53. From Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2017-06-14
Question
My boss were satisfied with the WB result of your anti-p53 Rabbit Monoclonal antibody. However we have observed positive staining in ovarian adenocarcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
B. Johnson
Verified customer
Asked: 2013-12-30
Answer
Based on literature, ovarian adenocarcinoma does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2013-12-30
