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Product Info Summary
| SKU: | EKC2086 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
MDM2 (Phospho-Ser166) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2086
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The MDM2 (Phospho-Ser166) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MDM2 (Phospho-Ser166) protein expression profile in cells. The kit can be used for measuring the relative amounts of MDM2 (Phospho-Ser166) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MDM2 (Phospho-Ser166).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
MDM2 (Phospho-Ser166) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2086)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2086 is reactive to MDM2 in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2086 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-MDM2 Phospho-Ser166 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-MDM2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COS7 cells
Protein Target Info & Infographic
Gene/Protein Information For MDM2 (Source: Uniprot.Org, NCBI)
Gene Name
MDM2
Full Name
E3 ubiquitin-protein ligase Mdm2
Weight
55233 MW
Superfamily
MDM2/MDM4 family
Alternative Names
Double minute 2 protein;HDM2;Oncoprotein Mdm2;p53-binding protein Mdm2;Ubiquitin-protein ligase E3 Mdm2 MDM2 ACTFS, HDMX, LSKB, hdm2 MDM2 proto-oncogene E3 ubiquitin-protein ligase Mdm2|MDM2 oncogene, E3 ubiquitin protein ligase|MDM2 proto-oncogene, E3 ubiquitin protein ligase|Mdm2, p53 E3 ubiquitin protein ligase homolog|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|oncoprotein Mdm2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MDM2, check out the MDM2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MDM2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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