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Product Info Summary
| SKU: | A00999-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GNS Antibody Picoband®
SKU/Catalog Number
A00999-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GNS Antibody Picoband® catalog # A00999-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GNS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00999-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human GNS recombinant protein (Position: W238-R355).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00999-1 is reactive to GNS in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
62.082kDa
Background of GNS
N-acetylglucosamine-6-sulfatase, also known as glucosamine (N-acetyl)-6-sulfatase, is an enzyme that in humans is encoded by the GNS gene. The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00999-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human PANC-1 whole cell, human SGC-7901 whole cell, rat lung tissue, rat kidney tissue, rat testis tissue, rat PC-12 whole cell, mouse lung tissue, mouse kidney tissue, mouse testis tissue, mouse spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GNS using anti-GNS antibody (A00999-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates,
Lane 3: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody (Catalog # A00999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.
Click image to see more details
Figure 2. Western blot analysis of GNS using anti-GNS antibody (A00999-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse spleen tissue lysates,
Lane 9: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody (Catalog # A00999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.
Protein Target Info & Infographic
Gene/Protein Information For GNS (Source: Uniprot.org, NCBI)
Gene Name
GNS
Full Name
N-acetylglucosamine-6-sulfatase
Weight
62.082kDa
Superfamily
sulfatase family
Alternative Names
N-acetylglucosamine-6-sulfatase; Glucosamine-6-sulfatase; G6S; GNS GNS G6S glucosamine (N-acetyl)-6-sulfatase N-acetylglucosamine-6-sulfatase|glucosamine -6-sulfatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNS, check out the GNS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GNS Antibody Picoband® (A00999-1)
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Customer Q&As
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7 Customer Q&As for Anti-GNS Antibody Picoband®
Question
I have attached the WB image, lot number and protocol we used for liver using anti-GNS antibody A00999-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-17
Question
Will anti-GNS antibody A00999-1 work on horse ELISA with testis?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Our lab technicians have not validated anti-GNS antibody A00999-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GNS antibody A00999-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse testis in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-GNS antibody A00999-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-GNS antibody (A00999-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
Question
My question regarding product A00999-1, anti-GNS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Krishna
Verified customer
Asked: 2017-10-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00999-1 anti-GNS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-03
Question
Does anti-GNS antibody A00999-1 work for WB with liver?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
According to the expression profile of liver, GNS is highly expressed in liver. So, it is likely that anti-GNS antibody A00999-1 will work for WB with liver.
Boster Scientific Support
Answered: 2017-10-02
Question
I am interested in to test anti-GNS antibody A00999-1 on rat liver for research purposes, then I may be interested in using anti-GNS antibody A00999-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Moore
Verified customer
Asked: 2017-04-19
Answer
The products we sell, including anti-GNS antibody A00999-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-19
Question
Is there a BSA free version of anti-GNS antibody A00999-1 available?
V. Anderson
Verified customer
Asked: 2015-12-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GNS antibody A00999-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-22
