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Product Info Summary
| SKU: | M01520-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381
SKU/Catalog Number
M01520-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381 (Catalog # M01520-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01520-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM381
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to C-terminus of human Matrix metalloproteinase-12 (MMP-12)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Matrix metalloproteinase-12 (MMP-12)
Reactive Species
M01520-1 is reactive to MMP12 in Human
Reconstitution
Calculated molecular weight
54002 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01520-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2500 dilution
WB: 1:200-1:1000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HEK 293 cells lysate using anti-MMP-12 rabbit monoclonal antibody (Clone RM381) at a 1:500 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-MMP-12 rabbit monoclonal antibody (Clone RM381) at a 1:2500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MMP12 (Source: Uniprot.org, NCBI)
Gene Name
MMP12
Full Name
Macrophage metalloelastase
Weight
54002 MW
Superfamily
peptidase M10A family
Alternative Names
Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP12, check out the MMP12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381 (M01520-1)
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Customer Q&As
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16 Customer Q&As for Anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381
Question
Is a blocking peptide available for product anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 (M01520-1)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We do provide the blocking peptide for product anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 (M01520-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-31
Question
I see that the anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 works with WB, what is the protocol used to produce the result images on the product page?
R. Rodriguez
Verified customer
Asked: 2019-12-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-10
Question
Will anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 work for WB with esophagus?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
According to the expression profile of esophagus, MMP12 is highly expressed in esophagus. So, it is likely that anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 will work for WB with esophagus.
Boster Scientific Support
Answered: 2019-09-11
Question
Would M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
As indicated on the product datasheet, M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-12
Question
We have seen staining in human alveolar macrophage. Do you have any suggestions? Is anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 supposed to stain alveolar macrophage positively?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
From what I have seen in literature alveolar macrophage does express MMP12. From what I have seen in Uniprot.org, MMP12 is expressed in amniotic fluid, alveolar macrophage, esophagus, among other tissues. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-01
Question
Is this M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 reactive to the isotypes of MMP12?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The immunogen of M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 is A peptide corresponding to C-terminus of human Matrix metalloproteinase-12 (MMP-12). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-22
Question
I was wanting to use your anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 for WB for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
It shows on the product datasheet, M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
Our lab were satisfied with the WB result of your anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
From what I have seen in literature, amniotic fluid does express MMP12. Generally MMP12 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
R. Wu
Verified customer
Asked: 2018-08-17
Answer
The anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 (M01520-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-17
Question
We ordered your anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
I looked at the website and datasheets of our anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-03-22
Question
Here is the WB image, lot number and protocol we used for esophagus using anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
Question
I am interested in to test anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 on human esophagus for research purposes, then I may be interested in using anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Li
Verified customer
Asked: 2017-01-05
Answer
The products we sell, including anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-05
Question
I have a question about product M01520-1, anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Rodriguez
Verified customer
Asked: 2015-12-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-12-22
Question
I would like using your anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 for negative regulation of type i interferon-mediated signaling pathway studies. Has this antibody been tested with western blotting on hek 293 cells? We would like to see some validation images before ordering.
D. Williams
Verified customer
Asked: 2014-07-08
Answer
We appreciate your inquiry. This M01520-1 anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 is tested on hek 293 cells, lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-08
Question
Do you have a BSA free version of anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 available?
G. Patel
Verified customer
Asked: 2013-06-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal antibody, Clone#RM381 M01520-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-06-06
