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Product Info Summary
| SKU: | PA1866 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband®
View all TGF-beta RII Antibodies
SKU/Catalog Number
PA1866
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TGF beta Receptor II/TGFBR2 Antibody catalog # PA1866. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1866)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TGF beta Receptor II, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1866 is reactive to TGFBR2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
64568 MW
Background of TGF-beta RII
TGFBR2 (transforming growth factor, beta receptor II (70/80kDa)), also known as TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II (TGF-beta receptor type II, TbetaR-II), is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. A TGFBR2 cDNA encoding a deduced 565-amino acid protein with a calculated molecular mass of approximately 60 kD in length. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. TGFBR2 may be a target of the EWS-FLI1 fusion protein found in Ewing sarcoma and related peripheral primitive neuroectodermal tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1866 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HEPG2 Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-TGF beta Receptor II antibody, PA1866, Western blotting
All lanes: Anti TGF beta Receptor II (PA1866) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
Protein Target Info & Infographic
Gene/Protein Information For TGFBR2 (Source: Uniprot.org, NCBI)
Gene Name
TGFBR2
Full Name
TGF-beta receptor type-2
Weight
64568 MW
Superfamily
protein kinase superfamily
Alternative Names
TGF-beta receptor type-2;TGFR-2;2.7.11.30;TGF-beta type II receptor;Transforming growth factor-beta receptor type II;TGF-beta receptor type II;TbetaR-II;TGFBR2; TGFBR2 AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII transforming growth factor beta receptor 2 TGF-beta receptor type-2|TGF-beta receptor type IIB|TGF-beta type II receptor|tbetaR-II|transforming growth factor beta receptor II|transforming growth factor beta receptor type IIC|transforming growth factor, beta receptor II (70/80kDa)|transforming growth factor, beta receptor II alpha|transforming growth factor, beta receptor II beta|transforming growth factor, beta receptor II delta|transforming growth factor, beta receptor II epsilon|transforming growth factor, beta receptor II gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFBR2, check out the TGFBR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFBR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband® (PA1866)
Hello CJ!
PA1866 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
Mechanism Investigation of the Improvement of Chang Run Tong on the Colonic Remodeling in Streptozotocin-Induced Diabetic Rats
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband®
Question
Is this PA1866 anti-TGF beta Receptor II/TGFBR2 antibody reactive to the isotypes of TGFBR2?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
The immunogen of PA1866 anti-TGF beta Receptor II/TGFBR2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human TGF beta Receptor II(330-346aa KGNLQEYLTRHVISWED), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-15
Question
We have seen staining in human liver. Are there any suggestions? Is anti-TGF beta Receptor II/TGFBR2 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
From what I have seen in literature liver does express TGFBR2. From what I have seen in Uniprot.org, TGFBR2 is expressed in metanephric glomerulus, liver, glial cell, placenta, cervix carcinoma, among other tissues. Regarding which tissues have TGFBR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 23186163
Glial cell, Pubmed ID: 7959019
Liver, Pubmed ID: 1310899, 8973329, 24275569
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-13
Question
you antibody to test anti-TGF beta Receptor II/TGFBR2 antibody PA1866 on mouse glial cell for research purposes, then I may be interested in using anti-TGF beta Receptor II/TGFBR2 antibody PA1866 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The products we sell, including anti-TGF beta Receptor II/TGFBR2 antibody PA1866, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-09
Question
Would anti-TGF beta Receptor II/TGFBR2 antibody PA1866 work on pig WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Our lab technicians have not validated anti-TGF beta Receptor II/TGFBR2 antibody PA1866 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TGF beta Receptor II/TGFBR2 antibody PA1866 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-18
Question
We are interested in using your anti-TGF beta Receptor II/TGFBR2 antibody for vasculogenesis studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thanks for your inquiry. This PA1866 anti-TGF beta Receptor II/TGFBR2 antibody is tested on hepg2 whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-14
Question
Here is the WB image, lot number and protocol we used for glial cell using anti-TGF beta Receptor II/TGFBR2 antibody PA1866. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
Does anti-TGF beta Receptor II/TGFBR2 antibody PA1866 work for WB with glial cell?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of glial cell, TGFBR2 is highly expressed in glial cell. So, it is likely that anti-TGF beta Receptor II/TGFBR2 antibody PA1866 will work for WB with glial cell.
Boster Scientific Support
Answered: 2019-08-12
Question
Is a blocking peptide available for product anti-TGF beta Receptor II/TGFBR2 antibody (PA1866)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
We do provide the blocking peptide for product anti-TGF beta Receptor II/TGFBR2 antibody (PA1866). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for glial cell using anti-TGF beta Receptor II/TGFBR2 antibody PA1866. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-13
Question
Can you help my question with product PA1866, anti-TGF beta Receptor II/TGFBR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Rodriguez
Verified customer
Asked: 2019-04-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1866 anti-TGF beta Receptor II/TGFBR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-29
Question
Is there a BSA free version of anti-TGF beta Receptor II/TGFBR2 antibody PA1866 available?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TGF beta Receptor II/TGFBR2 antibody PA1866 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-24
Question
We are currently using anti-TGF beta Receptor II/TGFBR2 antibody PA1866 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
F. Jones
Verified customer
Asked: 2018-06-29
Answer
The anti-TGF beta Receptor II/TGFBR2 antibody (PA1866) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-29
Question
Will PA1866 anti-TGF beta Receptor II/TGFBR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
It shows on the product datasheet, PA1866 anti-TGF beta Receptor II/TGFBR2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-06
Question
My boss were well pleased with the WB result of your anti-TGF beta Receptor II/TGFBR2 antibody. However we have seen positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is TGFBR2 supposed to be expressed?
S. Banerjee
Verified customer
Asked: 2017-04-19
Answer
According to literature, liver does express TGFBR2. Generally TGFBR2 expresses in cell membrane. Regarding which tissues have TGFBR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 23186163
Glial cell, Pubmed ID: 7959019
Liver, Pubmed ID: 1310899, 8973329, 24275569
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-04-19
Question
I see that the anti-TGF beta Receptor II/TGFBR2 antibody PA1866 works with WB, what is the protocol used to produce the result images on the product page?
T. Miller
Verified customer
Asked: 2014-04-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-03
Question
I was wanting to use your anti-TGF beta Receptor II/TGFBR2 antibody for WB for mouse glial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse glial cell identification?
R. Huang
Verified customer
Asked: 2013-01-03
Answer
You can see on the product datasheet, PA1866 anti-TGF beta Receptor II/TGFBR2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse glial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-03
