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Product Info Summary
| SKU: | M01520 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MMP12 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01520
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP12 Rabbit Monoclonal Antibody catalog # M01520. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP12 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01520)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CHE-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP12
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01520 is reactive to MMP12 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
54002 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01520 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of MMP12 expression in WI-38 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For MMP12 (Source: Uniprot.org, NCBI)
Gene Name
MMP12
Full Name
Macrophage metalloelastase
Weight
54002 MW
Superfamily
peptidase M10A family
Alternative Names
Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP12, check out the MMP12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP12 Rabbit Monoclonal Antibody (M01520)
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No publications found for M01520
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Recommended Resources
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Customer Q&As
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15 Customer Q&As for Anti-MMP12 Rabbit Monoclonal Antibody
Question
My colleagues were content with the WB result of your anti-MMP12 Rabbit Monoclonal antibody. However we have observed positive staining in amniotic fluid extracellular space using this antibody. Is that expected? Could you tell me where is MMP12 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
According to literature, amniotic fluid does express MMP12. Generally MMP12 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-21
Question
We are currently using anti-MMP12 Rabbit Monoclonal antibody M01520 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-MMP12 Rabbit Monoclonal antibody (M01520) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Is this M01520 anti-MMP12 Rabbit Monoclonal antibody reactive to the isotypes of MMP12?
V. Evans
Verified customer
Asked: 2019-10-15
Answer
The immunogen of M01520 anti-MMP12 Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP12. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-15
Question
Does anti-MMP12 Rabbit Monoclonal antibody M01520 work on feline WB with alveolar macrophage?
T. Brown
Verified customer
Asked: 2019-08-28
Answer
Our lab technicians have not validated anti-MMP12 Rabbit Monoclonal antibody M01520 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MMP12 Rabbit Monoclonal antibody M01520 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline alveolar macrophage in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-28
Question
We have seen staining in human amniotic fluid. What should we do? Is anti-MMP12 Rabbit Monoclonal antibody supposed to stain amniotic fluid positively?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Based on literature amniotic fluid does express MMP12. Based on Uniprot.org, MMP12 is expressed in amniotic fluid, alveolar macrophage, esophagus, among other tissues. Regarding which tissues have MMP12 expression, here are a few articles citing expression in various tissues:
Alveolar macrophage, Pubmed ID: 8226919
Esophagus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-31
Question
I see that the anti-MMP12 Rabbit Monoclonal antibody M01520 works with WB, what is the protocol used to produce the result images on the product page?
T. Wu
Verified customer
Asked: 2019-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-MMP12 Rabbit Monoclonal antibody M01520. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-04
Question
My question regarding product M01520, anti-MMP12 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01520 anti-MMP12 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-14
Question
I was wanting to use your anti-MMP12 Rabbit Monoclonal antibody for WB for human esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagus identification?
R. Williams
Verified customer
Asked: 2018-09-17
Answer
As indicated on the product datasheet, M01520 anti-MMP12 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-17
Question
Is there a BSA free version of anti-MMP12 Rabbit Monoclonal antibody M01520 available?
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP12 Rabbit Monoclonal antibody M01520 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-31
Question
Please see the WB image, lot number and protocol we used for esophagus using anti-MMP12 Rabbit Monoclonal antibody M01520. Please let me know if you require anything else.
E. Jones
Verified customer
Asked: 2015-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-23
Question
I would like to test anti-MMP12 Rabbit Monoclonal antibody M01520 on human esophagus for research purposes, then I may be interested in using anti-MMP12 Rabbit Monoclonal antibody M01520 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Kulkarni
Verified customer
Asked: 2015-01-19
Answer
The products we sell, including anti-MMP12 Rabbit Monoclonal antibody M01520, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-01-19
Question
Is a blocking peptide available for product anti-MMP12 Rabbit Monoclonal antibody (M01520)?
V. Moore
Verified customer
Asked: 2014-05-12
Answer
We do provide the blocking peptide for product anti-MMP12 Rabbit Monoclonal antibody (M01520). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-05-12
Question
Does anti-MMP12 Rabbit Monoclonal antibody M01520 work for WB with esophagus?
D. Evans
Verified customer
Asked: 2013-09-24
Answer
According to the expression profile of esophagus, MMP12 is highly expressed in esophagus. So, it is likely that anti-MMP12 Rabbit Monoclonal antibody M01520 will work for WB with esophagus.
Boster Scientific Support
Answered: 2013-09-24
Question
Would M01520 anti-MMP12 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Zhao
Verified customer
Asked: 2013-06-13
Answer
It shows on the product datasheet, M01520 anti-MMP12 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-13
