Product Info Summary
SKU: | A00421-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC |
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Product info
Product Name
Anti-IL-17A/Il17a Antibody Picoband™
View all IL-17/IL-17A Antibodies
SKU/Catalog Number
A00421-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-17A/Il17a Antibody Picoband™ catalog # A00421-3. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IL-17A/Il17a Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00421-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IL-17A/Il17a recombinant protein (Position: A18-S150).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00421-3 is reactive to IL17A in Mouse, Rat
Applications
A00421-3 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
27 kDa
Calculated molecular weight
Background of IL-17/IL-17A
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of IL-17A/Il17a using anti-IL-17A/Il17a antibody (A00421-3).
IL-17A/Il17a was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-17A/Il17a Antibody (A00421-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of IL-17A/Il17a using anti-IL-17A/Il17a antibody (A00421-3).
IL-17A/Il17a was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-17A/Il17a Antibody (A00421-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IL17A (Source: Uniprot.org, NCBI)
Gene Name
IL17A
Full Name
Interleukin-17A
Weight
Superfamily
IL-17 family
Alternative Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL17A, check out the IL17A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL17A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-17A/Il17a Antibody Picoband™ (A00421-3)
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Customer Reviews
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1 Reviews For Anti-IL-17A/Il17a Antibody Picoband™
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"Very happy with this product"
Excellent
Source: Customer Feedback Submission
"Very happy with this product"
--Ailin Lepletier de Oliveira
Application | IHC |
---|---|
Sample | Mouse spleen and lung |
"Very happy with this product. We were using an IL-17A from Abcam but found very high endogenous non-specific staining. This product was a much better stain in comparison. We used this antibody (1:200) on the Leica BOND Rxm autostainer, blocked with Sniper/1% BSA and it worked beautifully."
Ailin Lepletier de Oliveira
Verified customer
Submitted 2022-12-30
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